ArticleThe Biochemical journal2012
Location and dynamics of an active promoter in Escherichia coli K-12.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Dynamic plasmid clustering in Bacteria: Influence of transcriptional activity and host cellular states.Biochemistry and biophysics reports · 2025Article
- Effects of spatial heterogeneity on bacterial genetic circuits.PLoS computational biology · 2020Article
- High-Copy-Number Plasmid Segregation-Single-Molecule Dynamics in Single Cells.Biophysical journal · 2019Article
- Extrachromosomal Nucleolus-Like Compartmentalization by a Plasmid-Borne Ribosomal RNA Operon and Its Role in Nucleoid Compaction.Frontiers in microbiology · 2018Article
- Development of a new fluorescent reporter:operator system: location of AraC regulated genes in Escherichia coli K-12.BMC microbiology · 2017Article
- Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity.Applied and environmental microbiology · 2015Article
- Chromosome position effects on gene expression in Escherichia coli K-12.Nucleic acids research · 2014Article
- RNA polymerase: in search of promoters.Annals of the New York Academy of Sciences · 2013Review
- Genome architecture and global gene regulation in bacteria: making progress towards a unified model?Nature reviews. Microbiology · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In the present paper, we report that transcription affects the location of a DNA target in Escherichia coli K-12. A strain whose chromosome had been engineered to encode a lac repressor-GFP (green fluorescent protein) fusion was used as a host for a low copy number plasmid that carries an array of five lac operator sites. Individual cells of this strain exhibited a diffuse fluorescence signal, suggesting that the plasmid is distributed throughout the cell cytoplasm. However, a derivative of this plasmid carrying a cloned constitutive promoter is targeted to a location at the edge of the nucleoid towards the pole of the host cell. We conclude that transcription from the cloned promoter is driving the location of the plasmid and that specific locations in bacterial cells may favour gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.