ArticleACS nano2011
Detection of single enzymatic events in rare or single cells using microfluidics.
Article in ACS nano, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- Single-Cell Phenotypic Analysis and Digital Molecular Detection Linkable by a Hydrogel Bead-Based Platform.ACS applied bio materials · 2021Article
- Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker.Scientific reports · 2018Article
- The Effects of Dithiothreitol on DNA.Sensors (Basel, Switzerland) · 2017Article
- High-throughput screening of microchip-synthesized genes in programmable double-emulsion droplets.Nanoscale · 2017Article
- A versatile platform for surface modification of microfluidic droplets.Lab on a chip · 2017Article
- Biocompatibility of fluids for multiphase drops-in-drops microfluidics.Biomedical microdevices · 2016Article
- NanoCluster Beacons as reporter probes in rolling circle enhanced enzyme activity detection.Nanoscale · 2015Article
- Article
- Rare cell isolation and analysis in microfluidics.Lab on a chip · 2014Review
- Article
- Rapid formation of multicellular spheroids in double-emulsion droplets with controllable microenvironment.Scientific reports · 2013Article
- A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.Biomaterials · 2013Article
- Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.Analytical chemistry · 2013Review
- Article
- Flow-based systems for rapid and high-precision enzyme kinetics studies.Journal of analytical methods in chemistry · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
In the present study we demonstrate highly sensitive detection of rare, aberrant cells in a population of wild-type human cells by combining a rolling-circle-enhanced enzyme activity single-molecule detection assay with a custom-designed microfluidic device. Besides reliable detection of low concentrations of aberrant cells, the integrated system allowed multiplexed detection of individual enzymatic events at the single cell level. The single cell sensitivity of the presented setup relies on the combination of single-molecule rolling-circle-enhanced enzyme activity detection with the fast reaction kinetics provided by a picoliter droplet reaction volume and subsequent concentration of signals in a customized drop-trap device. This setup allows the fast reliable analyses of enzyme activities in a vast number of single cells, thereby offering a valuable tool for basic research as well as theranostics.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.