ArticleJournal of proteome research2011
Characterization of the human submandibular/sublingual saliva glycoproteome using lectin affinity chromatography coupled to multidimensional protein identification technology.
Article in Journal of proteome research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed.
- Aberrant Fucosylation of Saliva Glycoprotein Defining Lung Adenocarcinomas Malignancy.ACS omega · 2022Article
- Novel impacts of saliva with regard to oral health.The Journal of prosthetic dentistry · 2022Review
- Abnormal sialylation and fucosylation of saliva glycoproteins: Characteristics of lung cancer-specific biomarkers.Current research in pharmacology and drug discovery · 2022Review
- HBFP: a new repository for human body fluid proteome.Database : the journal of biological databases and curation · 2021Article
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- Review
- Salivary proteomics of healthy dogs: An in depth catalog.PloS one · 2018Article
- Construction and characterization of the Korean whole saliva proteome to determine ethnic differences in human saliva proteome.PloS one · 2017Article
- Simultaneous Enrichment of Plasma Soluble and Extracellular Vesicular Glycoproteins Using Prolonged Ultracentrifugation-Electrostatic Repulsion-hydrophilic Interaction Chromatography (PUC-ERLIC) Approach.Molecular & cellular proteomics : MCP · 2015Article
- Capturing and identification of differentially expressed fucome by a gel free and label free approach.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2015Article
- Gene expression in salivary glands: effects of diet and mouse chromosome 17 locus regulating macronutrient intake.Physiological reports · 2015Article
- Mass spectrometry-based analyses showing the effects of secretor and blood group status on salivary N-glycosylation.Clinical proteomics · 2015Article
- Review
- Article
- High-sensitivity analytical approaches for the structural characterization of glycoproteins.Chemical reviews · 2013Review
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- Analytical glycobiology at high sensitivity: current approaches and directions.Glycoconjugate journal · 2013Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In-depth analysis of the salivary proteome is fundamental to understanding the functions of salivary proteins in the oral cavity and to reveal disease biomarkers involved in different pathophysiological conditions, with the ultimate goal of improving patient diagnosis and prognosis. Submandibular and sublingual glands contribute saliva rich in glycoproteins to the total saliva output, making them valuable sources for glycoproteomic analysis. Lectin-affinity chromatography coupled to mass spectrometry-based shotgun proteomics was used to explore the submandibular/sublingual (SM/SL) saliva glycoproteome. A total of 262 N- and O-linked glycoproteins were identified by multidimensional protein identification technology (MudPIT). Only 38 were previously described in SM and SL salivas from the human salivary N-linked glycoproteome, while 224 were unique. Further comparison analysis with SM/SL saliva of the human saliva proteome, revealed 125 glycoproteins not formerly reported in this secretion. KEGG pathway analyses demonstrated that many of these glycoproteins are involved in processes such as complement and coagulation cascades, cell communication, glycosphingolipid biosynthesis neo-lactoseries, O-glycan biosynthesis, glycan structures-biosynthesis 2, starch and sucrose metabolism, peptidoglycan biosynthesis or others pathways. In summary, lectin-affinity chromatography coupled to MudPIT mass spectrometry identified many novel glycoproteins in SM/SL saliva. These new additions to the salivary proteome may prove to be a critical step for providing reliable biomarkers in the diagnosis of a myriad of oral and systemic diseases.
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Registered trials
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