ArticleFunctional & integrative genomics2012
Virus-induced gene silencing (VIGS) of genes expressed in root, leaf, and meiotic tissues of wheat.
Article in Functional & integrative genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 91 citations in OpenAlex.
- Functional validation of OsRPM1 as a positive regulator of bacterial blight resistance in rice via virus-induced gene silencing.Folia microbiologica · 2026Article
- APathogens (Basel, Switzerland) · 2025Article
- Wheat Tiller -1 (WT-1), a GRAS domain encoding gene, controls both tillering and spikelet number per spike in wheat.Plant molecular biology · 2025Article
- Rapid and Visual Screening of Virus Infection in Sugar Beets Through Polerovirus-Induced Gene Silencing.Viruses · 2024Article
- Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.Plant methods · 2024Article
- A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings.Horticulture research · 2024Article
- Applications of Virus-Induced Gene Silencing in Cotton.Plants (Basel, Switzerland) · 2024Review
- Establishment and application of a root wounding-immersion method for efficient virus-induced gene silencing in plants.Frontiers in plant science · 2024Article
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus.Plants (Basel, Switzerland) · 2022Article
- Knock-down of gene expression throughout meiosis and pollen formation by virus-induced gene silencing in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.).Plant methods · 2022Article
- Plant biomacromolecule delivery methods in the 21st century.Frontiers in genome editing · 2022Review
- Article
- Barley Stripe Mosaic Virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes in Wheat and Barley.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Virus-Induced Gene Silencing in Wheat and Related Monocot Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting.BMC plant biology · 2021Article
- An Efficient Virus-Induced Gene Silencing System for Functional Genomics Research in Walnut (Frontiers in plant science · 2021Article
- An EfficientFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barley stripe mosaic virus (BSMV)-based virus-induced gene silencing (VIGS) is an effective strategy for rapid functional analysis of genes in wheat leaves, but its utility to transiently express genes, and silencing in other tissues including root, flower, and developing grains, has not been demonstrated in monocots. We monitored green fluorescent protein (GFP) expression to demonstrate the utility of BSMV as a transient expression vector and silenced genes in various wheat tissues to expand VIGS utility to characterize tissue-specific genes. An antisense construct designed for coronatine insensitive1 (COI1) showed an 85% decrease in COI1 transcript level in roots accompanied by a 26% reduction in root length. Similarly, silencing of seed-specific granule-bound starch synthase by antisense and hairpin constructs resulted in up to 82% reduction in amylose content of the developing grains. VIGS of meiosis-specific genes demonstrated by silencing wheat homologue of disrupted meiosis cDNA1 (DMC1) by an antisense construct resulted in a 75-80% reduction in DMC1 transcript level accompanied by an average of 37.2 univalents at metaphase I. The virus-based transient GFP expression was observed in the leaf, phloem, and root cortex at 10-17 days post-inoculation. A novel observation was made that 8-11% of the first selfed generation progeny showed VIGS inheritance and that this proportion increased to 53-72% in the second and to 90-100% in the third generations. No viral symptoms were observed in the progeny, making it possible to study agronomic traits by VIGS. VIGS inheritance is particularly useful to study genes expressing during seed germination or other stages of early plant growth.
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