ArticleMolecular bioSystems2011
Molecular imaging of Cathepsin E-positive tumors in mice using a novel protease-activatable fluorescent probe.
Article in Molecular bioSystems, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Potential of uPAR, αvβ6 Integrin, and Tissue Factor as Targets for Molecular Imaging of Oral Squamous Cell Carcinoma: Evaluation of Nine Targets in Primary Tumors and Metastases by Immunohistochemistry.International journal of molecular sciences · 2023Article
- Review
- Cathepsin E expression and activity: Role in the detection and treatment of pancreatic cancer.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] · 2019Review
- Review
- Imaging the Tumor Microenvironment.Advances in experimental medicine and biology · 2017Review
- Optical imaging probes in oncology.Oncotarget · 2016Review
- Monitoring pancreatic carcinogenesis by the molecular imaging of cathepsin E in vivo using confocal laser endomicroscopy.PloS one · 2014Article
- Targeting cathepsin E in pancreatic cancer by a small molecule allows in vivo detection.Neoplasia (New York, N.Y.) · 2013Article
- Pancreatic cancer-associated Cathepsin E as a drug activator.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Designing and developing S100P inhibitor 5-methyl cromolyn for pancreatic cancer therapy.Molecular cancer therapeutics · 2013Article
- Article
- Activatable near-infrared fluorescent probe for in vivo imaging of fibroblast activation protein-alpha.Bioconjugate chemistry · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
unlabelledThe purpose of this study is to demonstrate the ability of imaging Cathepsin E (Cath E) positive tumors in living animals through selective targeting of Cath E proteolytic activity using a sensitive molecular imaging agent.
methodsA peptide-based Cath E imaging probe and a control probe were synthesized for this study. Human Cath E-positive cancer cells (MPanc96-E) were implanted subcutaneously in nude mice. Tumor-bearing mice were examined in vivo with near-infrared fluorescence (NIRF) imaging at various time points after intravenous injection of the Cath E sensing imaging probe. Excised organs and tissues of interest were further imaged ex vivo.
resultsUpon specific Cath E proteolytic activation, the NIRF signal of the imaging probe a was converted from an optically quenched initial state to a highly fluorescent active state. Imaging probe a was able to highlight the Cath E-positive tumors as early as 24 h post injection. Fluorescent signal in tumor was 3-fold higher than background. The confined specificity of imaging probe a to tumor associated Cath E was verified by using control imaging probe b. Both in vivo and ex vivo imaging results confirmed the superior selectivity and sensitivity of imaging probe a in Cath E imaging.
conclusionsThe small animal studies demonstrated the capability of probe a for imaging Cath E-positive tumors. The developed optical probe could be applied in early diagnostic imaging and guiding subsequent surgical procedure.
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