ArticleProceedings of the National Academy of Sciences of the United States of America2011
Protein interactions central to stabilizing the K+ channel selectivity filter in a four-sited configuration for selective K+ permeation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Mechanism of SK2 channel gating and its modulation by the bee toxin apamin and small molecules.eLife · 2025Article
- From Selective Permeation to Physiology in Potassium Channels.Function (Oxford, England) · 2025Review
- Article
- Ion and water permeation through claudin-10b and claudin-15 paracellular channels.Computational and structural biotechnology journal · 2024Article
- Cryo-EM structures of Kv1.2 potassium channels, conducting and non-conducting.bioRxiv : the preprint server for biology · 2024Article
- Molecular Events behind the Selectivity and Inactivation Properties of Model NaK-Derived Ion Channels.International journal of molecular sciences · 2022Article
- Structural Basis for pH-gating of the KNature communications · 2022Article
- Pore-forming transmembrane domains control ion selectivity and selectivity filter conformation in the KirBac1.1 potassium channel.The Journal of general physiology · 2021Article
- Structural Plasticity of the Selectivity Filter in Cation Channels.Frontiers in physiology · 2021Review
- The structure of a potassium-selective ion channel reveals a hydrophobic gate regulating ion permeation.IUCrJ · 2020Article
- Direct knock-on of desolvated ions governs strict ion selectivity in KNature chemistry · 2018Article
- The permeation mechanism of organic cations through a CNG mimic channel.PLoS computational biology · 2018Article
- Exploring the Viral Channel KcvThe Journal of membrane biology · 2018Article
- A single NaK channel conformation is not enough for non-selective ion conduction.Nature communications · 2018Article
- MicroED structure of the NaK ion channel reveals a NaCommunications biology · 2018Article
- Ion channels and ion selectivity.Essays in biochemistry · 2017Review
- Tuning the ion selectivity of two-pore channels.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Multi-ion free energy landscapes underscore the microscopic mechanism of ion selectivity in the KcsA channel.Biochimica et biophysica acta · 2016Article
- Role of protein dynamics in ion selectivity and allosteric coupling in the NaK channel.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Recent advances in engineering microbial rhodopsins for optogenetics.Current opinion in structural biology · 2015Review
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Authors and funding
4 authors.
Funding
Abstract
The structural and functional conversion of the nonselective NaK channel to a K(+) selective channel (NaK2K) allows us to identify two key residues, Tyr and Asp in the filter sequence of TVGYGD, that participate in interactions central to stabilizing the K(+) channel selectivity filter. By using protein crystallography and channel electrophysiology, we demonstrate that the K(+) channel filter exists as an energetically strained structure and requires these key protein interactions working in concert to hold the filter in the precisely defined four-sited configuration that is essential for selective K(+) permeation. Disruption of either interaction, as tested on both the NaK2K and eukaryotic K(v)1.6 channels, can reduce or completely abolish K(+) selectivity and in some cases may also lead to channel inactivation due to conformational changes at the filter. Additionally, on the scaffold of NaK we recapitulate the protein interactions found in the filter of the Kir channel family, which uses a distinct interaction network to achieve similar stabilization of the filter.
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