ArticleThe Biochemical journal2012
Vitamin E isoforms directly bind PKCα and differentially regulate activation of PKCα.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
- Associations of α- and γ-tocopherol during early life with lung function in childhood.The Journal of allergy and clinical immunology · 2020Trial
- The Neuroprotective Effects of Alpha-Tocopherol as an Anti-Inflammatory Agent: Mechanistic Insights and Therapeutic Challenges.Nutrients · 2026Review
- Nutrients and bioactive compounds as modifiers of neurodegenerative trajectories: molecular mechanisms, translational barriers, and precision nutrition.Frontiers in nutrition · 2026Review
- Correlation between vitamin A, D, E, and K levels in children with allergic rhinitis: a propensity-matched analysis.BMC pediatrics · 2025Article
- Maternal supplementation with α-tocopherol inhibits the development of offspring food allergy, H1R signaling and ultimately anaphylaxis early in life.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- The Role of Vitamins in the Pathogenesis of Asthma.International journal of molecular sciences · 2023Review
- Dysbiotic lung microbial communities of neonates from allergic mothers confer neonate responsiveness to suboptimal allergen.Frontiers in allergy · 2023Article
- β-Glucosylceramides and Tocopherols Regulate Development and Function of Dendritic Cells.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Asthma, allergy and vitamin E: Current and future perspectives.Free radical biology & medicine · 2022Article
- Vitamin E acetate as linactant in the pathophysiology of EVALI.Medical hypotheses · 2020Article
- α-Tocopherol Attenuates the Severity ofAmerican journal of respiratory cell and molecular biology · 2020Article
- Plasma versus Erythrocyte Vitamin E in Renal Transplant Recipients, and Duality of Tocopherol Species.Nutrients · 2019Article
- Association of physical activity, vitamin E levels, and total antioxidant capacity with academic performance and executive functions of adolescents.BMC pediatrics · 2019Article
- Fat-soluble vitamins A and E and health disparities in a cohort of pregnant women at delivery.Journal of nutritional science · 2018Article
- Oxidative Stress: Harms and Benefits for Human Health.Oxidative medicine and cellular longevity · 2017Review
- Genetic Variations Involved in Vitamin E Status.International journal of molecular sciences · 2016Review
- A Comparison of Nutritional Antioxidant Content in Breast Milk, Donor Milk, and Infant Formulas.Nutrients · 2016Article
- Article
- γ-Tocopherol supplementation of allergic female mice augments development of CD11c+CD11b+ dendritic cells in utero and allergic inflammation in neonates.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Excessive α-tocopherol exacerbates microglial activation and brain injury caused by acute ischemic stroke.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Vitamin E isoforms have opposing regulatory effects on leucocyte recruitment during inflammation. Furthermore, in vitro, vitamin E isoforms have opposing effects on leucocyte migration across endothelial cells by regulating VCAM (vascular cell-adhesion molecule)-1 activation of endothelial cell PKCα (protein kinase Cα). However, it is not known whether tocopherols directly regulate cofactor-dependent or oxidative activation of PKCα. We report in the present paper that cofactor-dependent activation of recombinant PKCα was increased by γ-tocopherol and was inhibited by α-tocopherol. Oxidative activation of PKCα was inhibited by α-tocopherol at a 10-fold lower concentration than γ-tocopherol. In binding studies, NBD (7-nitrobenz-2-oxa-1,3-diazole)-tagged α-tocopherol directly bound to full-length PKCα or the PKCα-C1a domain, but not PKCζ. NBD-tagged α-tocopherol binding to PKCα or the PKCα-C1a domain was blocked by diacylglycerol, α-tocopherol, γ-tocopherol and retinol, but not by cholesterol or PS (phosphatidylserine). Tocopherols enhanced PKCα-C2 domain binding to PS-containing lipid vesicles. In contrast, the PKCα-C2 domain did not bind to lipid vesicles containing tocopherol without PS. The PKCα-C1b domain did not bind to vesicles containing tocopherol and PS. In summary, α-tocopherol and γ-tocopherol bind the diacylglycerol-binding site on PKCα-C1a and can enhance PKCα-C2 binding to PS-containing vesicles. Thus the tocopherols can function as agonists or antagonists for differential regulation of PKCα.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.