ArticleJournal of molecular neuroscience : MN2012
P2X7 receptor-pannexin 1 hemichannel association: effect of extracellular calcium on membrane permeabilization.
Article in Journal of molecular neuroscience : MN, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Adenosine triphosphate release inhibitors targeting pannexin1 improve recovery after spinal cord injury.Nagoya journal of medical science · 2024Article
- The Pannexin 1 Channel and the P2X7 Receptor Are in Complex Interplay to Regulate the Release of Soluble Ectonucleotidases in the Murine Bladder Lamina Propria.International journal of molecular sciences · 2023Article
- The Impact of Plasma Membrane Ion Channels on Bone Remodeling in Response to Mechanical Stress, Oxidative Imbalance, and Acidosis.Antioxidants (Basel, Switzerland) · 2023Review
- Gap Junctions and Connexins in Microglia-Related Oxidative Stress and Neuroinflammation: Perspectives for Drug Discovery.Biomolecules · 2023Review
- Microglial pyroptosis: Therapeutic target in secondary brain injury following intracerebral hemorrhage.Frontiers in cellular neuroscience · 2022Review
- Astroglial Hemichannels and Pannexons: The Hidden Link between Maternal Inflammation and Neurological Disorders.International journal of molecular sciences · 2021Review
- Review
- P2X7 Interactions and Signaling - Making Head or Tail of It.Frontiers in molecular neuroscience · 2019Review
- The Opening of Connexin 43 Hemichannels Alters Hippocampal Astrocyte Function and Neuronal Survival in Prenatally LPS-Exposed Adult Offspring.Frontiers in cellular neuroscience · 2019Article
- Connexin and Pannexin-Based Channels in Oligodendrocytes: Implications in Brain Health and Disease.Frontiers in cellular neuroscience · 2019Review
- Folliculin Regulates Osteoclastogenesis Through Metabolic Regulation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2018Article
- Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model.Molecular medicine reports · 2018Article
- Interactions of Pannexin1 channels with purinergic and NMDA receptor channels.Biochimica et biophysica acta. Biomembranes · 2018Review
- D-serine released by astrocytes in brainstem regulates breathing response to CONature communications · 2017Article
- Article
- Inhibition of the P2X7-PANX1 complex suppresses spreading depolarization and neuroinflammation.Brain : a journal of neurology · 2017Article
- Pannexin- and Connexin-Mediated Intercellular Communication in Platelet Function.International journal of molecular sciences · 2017Review
- Regulation of Pannexin 1 Surface Expression by Extracellular ATP: Potential Implications for Nervous System Function in Health and Disease.Frontiers in cellular neuroscience · 2017Article
- Connexins and Pannexins: New Insights into Microglial Functions and Dysfunctions.Frontiers in molecular neuroscience · 2016Review
- The action of mimetic peptides on connexins protects fibroblasts from the negative effects of ischemia reperfusion.Biology open · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of P2X(7) receptor (P2X(7)R) and pannexin have been implicated in membrane permeabilization associated with ischemic cell death and many other inflammatory processes. P2X(7)R has a unique property of forming large pore upon repeated or prolonged application of agonist like ATP or 2', 3'-(4-benzoyl) benzoyl ATP. It has been proposed that pannexin 1 (panx1) hemichannel associates with P2X(7)R to form large pore, though the actual mechanism is not yet understood. Calcium concentration in extracellular milieu drops in many patho-physiological conditions, e.g. ischemia, when P2X(7)R/pannexin is also known to be activated. Therefore, we hypothesize that extracellular calcium ([Ca(2+)](o)) plays an important role in the coupling of P2X(7)R-panx1 and subsequent membrane permeabilization. In this study we show that membrane permeability of the P2X(7)R and panx1 expressing N2A cell increases in ([Ca(2+)](o))-free solution. In [Ca(2+)](o)-free solution, fluorescent dye calcein trapped cells exhibited time-dependent dye leakage resulting in about 50% decrease of fluorescence intensity in 30 min. Control cells in 2 mM [Ca(2+)](o) did not show such leakage. Like N2A cells, mixed culture of neuron and glia, derived from hippocampal progenitor cells showed similar dye leakage. Dye leakage was blocked either by pannexin-specific blocker, carbenoxolone or P2X(7)R antagonists, Brilliant Blue G, and oxidized ATP. Furthermore P2X(7)R and panx1 were co-immunoprecipitated. The amount of P2X(7)R protein pulled-down with panx1, increased by twofold when cells were incubated 30 min in [Ca(2+)](o)-free buffer. Taken together, the results of this study demonstrate the activation and association of P2X(7)R-panx1, triggered by the removal of [Ca(2+)](o).
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Registered trials
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