ArticlePloS one2011
In vitro interactions between bacteria, osteoblast-like cells and macrophages in the pathogenesis of biomaterial-associated infections.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 68 citations in OpenAlex.
- Balancing Osseointegration and Infection Control: The Role of Titanium Surface Topography in Peri-Implant Biology.Journal of functional biomaterials · 2026Article
- Fine-Tuned Functionalizable Terpolymer Brush Nanocoating Resists Protein Adsorption and Bacterial Adhesion while Promoting Macrophage Activity and Osteoblast Proliferation.ACS applied materials & interfaces · 2025Article
- Longitudinal intravital imaging of the bone marrow for analysis of the race for the surface in a murine osteomyelitis model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- The protective effect of apolipoprotein H in paediatric sepsis.Critical care (London, England) · 2024Article
- Evaluation of silver bio-functionality in a multicellularFrontiers in cellular and infection microbiology · 2023Article
- Antibacterial Designs for Implantable Medical Devices: Evolutions and Challenges.Journal of functional biomaterials · 2022Review
- The ratio of nicotinic acid to nicotinamide as a microbial biomarker for assessing cell therapy product sterility.Molecular therapy. Methods & clinical development · 2022Article
- The road to success of coagulase-negative staphylococci: clinical significance of small colony variants and their pathogenic role in persistent infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- Antibacterial effects of platelet-rich fibrin produced by horizontal centrifugation.International journal of oral science · 2020Article
- Three-DimensionalInfection and immunity · 2020Article
- Antibacterial and Osteogenic Functionalization of Titanium With Silicon/Copper-Doped High-Energy Shot Peening-Assisted Micro-Arc Oxidation Technique.Frontiers in bioengineering and biotechnology · 2020Article
- Co-culture of induced pluripotent stem cells with cardiomyocytes is sufficient to promote their differentiation into cardiomyocytes.PloS one · 2020Article
- Impact of nanosystems in Staphylococcus aureus biofilms treatment.FEMS microbiology reviews · 2019Review
- Co-Culture of S. epidermidis and Human Osteoblasts on Implant Surfaces: An Advanced In Vitro Model for Implant-Associated Infections.PloS one · 2016Article
- The role of programmed death ligand 1 pathway in persistent biomaterial-associated infections.Journal of microbiology (Seoul, Korea) · 2015Article
- Article
- A mucosal model to study microbial biofilm development and anti-biofilm therapeutics.Journal of microbiological methods · 2013Article
- Monitoring mitochondrial inner membrane potential for detecting early changes in viability of bacterium-infected human bone marrow-derived mesenchymal stem cells.Stem cell research & therapy · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biomaterial-associated infections constitute a major clinical problem that is difficult to treat and often necessitates implant replacement. Pathogens can be introduced on an implant surface during surgery and compete with host cells attempting to integrate the implant. The fate of a biomaterial implant depends on the outcome of this race for the surface. Here we studied the competition between different bacterial strains and human U2OS osteoblast-like cells (ATCC HTB-94) for a poly(methylmethacrylate) surface in the absence or presence of macrophages in vitro using a peri-operative contamination model. Bacteria were seeded on the surface at a shear rate of 11 1/s prior to adhesion of U2OS cells and macrophages. Next, bacteria, U2OS cells and macrophages were allowed to grow simultaneously under low shear conditions (0.14 1/s). The outcome of the competition between bacteria and U2OS cells for the surface critically depended on bacterial virulence. In absence of macrophages, highly virulent Staphylococcus aureus or Pseudomonas aeruginosa stimulated U2OS cell death within 18 h of simultaneous growth on a surface. Moreover, these strains also caused cell death despite phagocytosis of adhering bacteria in presence of murine macrophages. Thus U2OS cells are bound to loose the race for a biomaterial surface against S. aureus or P. aeruginosa, even in presence of macrophages. In contrast, low-virulent Staphylococcus epidermidis did not cause U2OS cell death even after 48 h, regardless of the absence or presence of macrophages. Clinically, S. aureus and P. aeruginosa are known to yield acute and severe biomaterial-associated infections in contrast to S. epidermidis, mostly known to cause more low-grade infection. Thus it can be concluded that the model described possesses features concurring with clinical observations and therewith has potential for further studies on the simultaneous competition for an implant surface between tissue cells and pathogenic bacteria in presence of immune system components.
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Registered trials
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