ArticlePloS one2011
Global gene expression analysis of fission yeast mutants impaired in Ser-2 phosphorylation of the RNA pol II carboxy terminal domain.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Multiple roles of CTDK-I throughout the cell.Cellular and molecular life sciences : CMLS · 2019Review
- Latrunculin A-Induced Perturbation of the Actin Cytoskeleton Mediates Pap1p-Dependent Induction of the Caf5p Efflux Pump inG3 (Bethesda, Md.) · 2017Article
- Phospho-site mutants of the RNA Polymerase II C-terminal domain alter subtelomeric gene expression and chromatin modification state in fission yeast.Nucleic acids research · 2016Article
- A Genetic Screen for Fission Yeast Gene Deletion Mutants Exhibiting Hypersensitivity to Latrunculin A.G3 (Bethesda, Md.) · 2016Article
- Article
- Individual letters of the RNA polymerase II CTD code govern distinct gene expression programs in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- On the computational ability of the RNA polymerase II carboxy terminal domain.Communicative & integrative biology · 2014Article
- Synthetically engineered rpb1 alleles altering RNA polymerase II carboxy terminal domain phosphorylation induce discrete morphogenetic defects in Schizosaccharomyces pombe.Communicative & integrative biology · 2013Article
- Decoding the informational properties of the RNA polymerase II Carboxy Terminal Domain.BMC research notes · 2012Article
- A conserved histone deacetylase with a role in the regulation of cytokinesis in Schizosaccharomyces pombe.Cell division · 2012Article
- Ensuring the faithful execution of cytokinesis in Schizosaccharomyces pombe.Communicative & integrative biology · 2012Article
- Rhn1, a nuclear protein, is required for suppression of meiotic mRNAs in mitotically dividing fission yeast.PloS one · 2012Article
- The SET domain protein, Set3p, promotes the reliable execution of cytokinesis in Schizosaccharomyces pombe.PloS one · 2012Article
- Distinct requirement of RNA polymerase II CTD phosphorylations in budding and fission yeast.TranscriptionArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Schizosaccharomyces pombe the nuclear-localized Lsk1p-Lsc1p cyclin dependent kinase complex promotes Ser-2 phosphorylation of the heptad repeats found within the RNA pol II carboxy terminal domain (CTD). Here, we first provide evidence supporting the existence of a third previously uncharacterized Ser-2 CTD kinase subunit, Lsg1p. As expected for a component of the complex, Lsg1p localizes to the nucleus, promotes Ser-2 phosphorylation of the CTD, and physically interacts with both Lsk1p and Lsc1p in vivo. Interestingly, we also demonstrate that lsg1Δ mutants--just like lsk1Δ and lsc1Δ strains--are compromised in their ability to faithfully and reliably complete cytokinesis. Next, to address whether kinase mediated alterations in CTD phosphorylation might selectively alter the expression of genes with roles in cytokinesis and/or the cytoskeleton, global gene expression profiles were analyzed. Mutants impaired in Ser-2 phosphorylation display little change with respect to the level of transcription of most genes. However, genes affecting cytokinesis--including the actin interacting protein gene, aip1--as well as genes with roles in meiosis, are included in a small subset that are differentially regulated. Significantly, genetic analysis of lsk1Δ aip1Δ double mutants is consistent with Lsk1p and Aip1p acting in a linear pathway with respect to the regulation of cytokinesis.
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