ArticlePloS one2011
Defining the earliest transcriptional steps of chondrogenic progenitor specification during the formation of the digits in the embryonic limb.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Species-specific roles of cellular communication network proteins in cartilage development: A comparative study using in vitro chondrogenic models.Journal of cell communication and signaling · 2026Article
- Epitenon-derived progenitors drive fibrosis and regeneration after flexor tendon injury in a spatially-dependent manner.Nature communications · 2025Article
- Neuronal Guidance Molecules in Bone Remodeling and Orthodontic Tooth Movement.International journal of molecular sciences · 2022Review
- Patterning of cartilaginous condensations in the developing facial skeleton.Developmental biology · 2022Article
- A Novel EphA4 Signaling-Based Therapeutic Strategy for Osteoarthritis in Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- BMP signaling and skeletal development in fibrodysplasia ossificans progressiva (FOP).Developmental dynamics : an official publication of the American Association of Anatomists · 2022Review
- Selection on Phalanx Development in the Evolution of the Bird Wing.Molecular biology and evolution · 2021Article
- Effects of Berberine on the Chondrogenic Differentiation of Embryonic Limb Skeletal Progenitors.Journal of inflammation research · 2021Article
- Eph-Ephrin Signaling Mediates Cross-Talk Within the Bone Microenvironment.Frontiers in cell and developmental biology · 2021Review
- The methylation status of the embryonic limb skeletal progenitors determines their cell fate in chicken.Communications biology · 2020Article
- Confluence of Cellular Degradation Pathways During Interdigital Tissue Remodeling in Embryonic Tetrapods.Frontiers in cell and developmental biology · 2020Review
- Understanding the Cellular and Molecular Mechanisms That Control Early Cell Fate Decisions During Appendicular Skeletogenesis.Frontiers in genetics · 2019Review
- The role of chondroitin sulfate in regulating hypertrophy during MSC chondrogenesis in a cartilage mimetic hydrogel under dynamic loading.Biomaterials · 2019Article
- Complexity: the organizing principle at the interface of biological (dis)order.Journal of genetics · 2017Review
- On the Formation of Digits and Joints during Limb Development.Developmental cell · 2017Article
- Article
- DNA damage precedes apoptosis during the regression of the interdigital tissue in vertebrate embryos.Scientific reports · 2016Article
- Deep phylogenomics of a tandem-repeat galectin regulating appendicular skeletal pattern formation.BMC evolutionary biology · 2016Article
- Mammalian tolloid proteinases: role in growth factor signalling.FEBS letters · 2016Review
- Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face.PLoS genetics · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The characterization of genes involved in the formation of cartilage is of key importance to improve cell-based cartilage regenerative therapies. Here, we have developed a suitable experimental model to identify precocious chondrogenic events in vivo by inducing an ectopic digit in the developing embryo. In this model, only 12 hr after the implantation of a Tgfβ bead, in the absence of increased cell proliferation, cartilage forms in undifferentiated interdigital mesoderm and in the course of development, becomes a structurally and morphologically normal digit. Systematic quantitative PCR expression analysis, together with other experimental approaches allowed us to establish 3 successive periods preceding the formation of cartilage. The "pre-condensation stage", occurring within the first 3 hr of treatment, is characterized by the activation of connective tissue identity transcriptional factors (such as Sox9 and Scleraxis) and secreted factors (such as Activin A and the matricellular proteins CCN-1 and CCN-2) and the downregulation of the galectin CG-8. Next, the "condensation stage" is characterized by intense activation of Smad 1/5/8 BMP-signaling and increased expression of extracellular matrix components. During this period, the CCN matricellular proteins promote the expression of extracellular matrix and cell adhesion components. The third period, designated the "pre-cartilage period", precedes the formation of molecularly identifiable cartilage by 2-3 hr and is characterized by the intensification of Sox 9 gene expression, along with the stimulation of other pro-chondrogenic transcription factors, such as HifIa. In summary, this work establishes a temporal hierarchy in the regulation of pro-chondrogenic genes preceding cartilage differentiation and provides new insights into the relative roles of secreted factors and cytoskeletal regulators that direct the first steps of this process in vivo.
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