Trial reportPloS one2011
A novel system for transcutaneous application of carbon dioxide causing an "artificial Bohr effect" in the human body.
Trial report in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04561609 (The Effect of Transcutaneous Application of Gaseous CO2 on Diabetic Chronic Wound Healing), which is not on this map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Effect of Transcutaneous Application of Gaseous CO2 on Diabetic Chronic Wound Healing
Who cites it
41 citing papers in PubMed, 94 citations in OpenAlex.
- The effects of bathing in neutral bicarbonate ion water.Scientific reports · 2021Trial
- The effect of transcutaneous application of gaseous COInternational wound journal · 2020Trial
- Transcutaneous Carbon Dioxide Therapy Significantly Accelerates Diabetic Foot Ulcer Healing: A Multicentre Randomised Controlled Trial.Biomedicines · 2026Article
- Effects of carbon dioxide hydrate cryotherapy on knee flexor muscle function recovery trajectory after muscle damage: a randomized controlled trial.Frontiers in physiology · 2026Article
- Hydrogels for Bone Regeneration: Properties, Additives, Preclinical and Clinical Applications.Gels (Basel, Switzerland) · 2025Review
- Synergistic COInternational journal of molecular sciences · 2025Article
- Potential therapeutic applications of medical gases in cancer treatment.Medical gas research · 2025Review
- Effect of Transcutaneous Application of Carbon Dioxide on Wound Healing, Wound Recurrence Rate and Diabetic Polyneuropathy in Patients with Neuropathic, Ischemic and Neuroischemic Diabetes-Related Foot Ulcers.Life (Basel, Switzerland) · 2025Article
- Effects of Carbon Dioxide Therapy on Skin Wound Healing.Biomedicines · 2025Review
- Effect of intermittent extracellular low-pH environment on human umbilical vein endothelial cell activity.PloS one · 2025Article
- Transcutaneous COBMC musculoskeletal disorders · 2024Article
- Transcutaneous carbon dioxide suppresses skeletal muscle atrophy in a mouse model of oral squamous cell carcinoma.PloS one · 2024Article
- Precooling via immersion in COTemperature (Austin, Tex.) · 2024Article
- Novel COScientific reports · 2023Article
- Effects of the Percutaneous Carbon Dioxide Therapy on Post-surgical and Post-traumatic Hematoma, Edema and Pain.Journal of orthopaedic case reports · 2023Article
- The Efficacy of Carbon Dioxide Paste in Alleviating Pain in Patients After Neck Dissection: Protocol for a Double-Blinded, Randomized Controlled Trial.JMIR research protocols · 2023Article
- Carbon dioxide and MAPK signalling: towards therapy for inflammation.Cell communication and signaling : CCS · 2023Review
- Local application of a transcutaneous carbon dioxide paste prevents excessive scarring and promotes muscle regeneration in a bupivacaine-induced rat model of muscle injury.International wound journal · 2023Article
- The Tissue Response to Hypoxia: How Therapeutic Carbon Dioxide Moves the Response toward Homeostasis and Away from Instability.International journal of molecular sciences · 2023Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCarbon dioxide (CO(2)) therapy refers to the transcutaneous administration of CO(2) for therapeutic purposes. This effect has been explained by an increase in the pressure of O(2) in tissues known as the Bohr effect. However, there have been no reports investigating the oxygen dissociation of haemoglobin (Hb) during transcutaneous application of CO(2)in vivo. In this study, we investigate whether the Bohr effect is caused by transcutaneous application of CO2 in human living body.
methodsWe used a novel system for transcutaneous application of CO(2) using pure CO(2) gas, hydrogel, and a plastic adaptor. The validity of the CO(2) hydrogel was confirmed in vitro using a measuring device for transcutaneous CO(2) absorption using rat skin. Next, we measured the pH change in the human triceps surae muscle during transcutaneous application of CO(2) using phosphorus-31 magnetic resonance spectroscopy ((31)P-MRS) in vivo. In addition, oxy- and deoxy-Hb concentrations were measured with near-infrared spectroscopy in the human arm with occulted blood flow to investigate O2 dissociation from Hb caused by transcutaneous application of CO(2).
resultsThe rat skin experiment showed that CO(2) hydrogel enhanced CO(2) gas permeation through the rat skin. The intracellular pH of the triceps surae muscle decreased significantly 10 min. after transcutaneous application of CO(2). The NIRS data show the oxy-Hb concentration decreased significantly 4 min. after CO(2) application, and deoxy-Hb concentration increased significantly 2 min. after CO(2) application in the CO(2)-applied group compared to the control group. Oxy-Hb concentration significantly decreased while deoxy-Hb concentration significantly increased after transcutaneous CO(2) application.
conclusionsOur novel transcutaneous CO(2) application facilitated an O(2) dissociation from Hb in the human body, thus providing evidence of the Bohr effect in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.