ArticlePloS one2011
Reliable Drosophila body fat quantification by a coupled colorimetric assay.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 94 citations in OpenAlex.
- Article
- Mitochondrial sirtuin 4 shapes the intestinal microbiota of Drosophila by controlling lysozyme expression.Animal microbiome · 2025Article
- Time-Restricted Feeding Promotes Longevity and Gut Health Without Fitness Trade-Offs.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Insulin/insulin-like growth factor signaling pathway promotes higher fat storage inbioRxiv : the preprint server for biology · 2025Article
- Adipocyte-DerivedBiomolecules · 2025Article
- Temperature-dependent dynamics of energy stores in Drosophila.BMC biology · 2024Article
- LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance.Nature communications · 2024Article
- Dietary sucrose determines the regulatory activity of lithium on gene expression and lifespan inAging · 2024Article
- Article
- A protein restricted diet induces a stable increased fat storage phenotype in flies.Toxicology reports · 2023Article
- The Drosophila melanogaster ACE2 ortholog genes are differently expressed in obesity/diabetes and aging models: Implications for COVID-19 pathology.Biochimica et biophysica acta. Molecular basis of disease · 2022Article
- A gut-derived hormone suppresses sugar appetite and regulates food choice in Drosophila.Nature metabolism · 2022Article
- Article
- Article
- An aqueous extract of the brown algaAging · 2022Article
- Anti-obesity effects ofHeliyon · 2022Article
- The gut hormone Allatostatin C/Somatostatin regulates food intake and metabolic homeostasis under nutrient stress.Nature communications · 2022Article
- Phenotyping ofBiomolecules · 2022Review
- Article
- Predicting novel candidate human obesity genes and their site of action by systematic functional screening in Drosophila.PLoS biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Factors and mechanisms controlling lipometabolism homeostasis share a remarkable evolutionary conservation between humans and Drosophila flies. Accordingly, the Drosophila model has been successfully used to understand the pathophysiology of human metabolic diseases such as obesity. Body fat stores in species as different as humans and flies consist of neutral lipids, mainly triacylglycerols. Changes in body fat storage are a diagnostic phenotype of lipometabolism imbalances of genetic or environmental origin. Various methods have been developed to quantify Drosophila body fat storage. The most widely used method adopts a commercial coupled colorimetric assay designed for human serum triacylglycerol quantification, which is based on glycerol content determination after enzymatic conversion of glycerides into glycerol. The coupled colorimetric assay is compatible with large-scale genetic screen approaches and has been successfully applied to characterize central regulators of Drosophila lipometabolism. Recently, the applicability of the coupled colorimetric assay for Drosophila storage fat quantification has been questioned in principle. Here we compare the performance of the coupled colorimetric assay on Drosophila samples with thin layer chromatography, the "gold standard" in storage lipid analysis. Our data show that the presented variant of the coupled colorimetric assay reliably discriminates between lean and fat flies and allows robust, quick and cost-effective quantification of Drosophila body fat stores.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.