ArticleActa crystallographica. Section D, Biological crystallography2011
Two-photon excited UV fluorescence for protein crystal detection.
Article in Acta crystallographica. Section D, Biological crystallography, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Single-cell structural biology with intracellular electron crystallography.Nature communications · 2026Article
- Non-invasive nanoscale imaging of protein micro- and nanocrystals for screening crystallization conditions.Journal of applied crystallography · 2024Article
- Reaching the potential of electron diffraction.Cell reports. Physical science · 2024Article
- High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography.Journal of visualized experiments : JoVE · 2023Article
- 20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening.Acta crystallographica. Section D, Structural biology · 2023Article
- Depth-of-field extension in optical imaging for rapid crystal screening.Acta crystallographica. Section D, Structural biology · 2021Article
- Article
- A fully automated crystallization apparatus for small protein quantities.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.Journal of applied crystallography · 2020Article
- Protein-crystal detection with a compact multimodal multiphoton microscope.Communications biology · 2020Article
- Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Acta crystallographica. Section F, Structural biology communications · 2019Article
- DeepCentering: fully automated crystal centering using deep learning for macromolecular crystallography.Journal of synchrotron radiation · 2019Article
- Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer.Acta crystallographica. Section F, Structural biology communications · 2018Article
- The complex analysis of X-ray mesh scans for macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2018Article
- A low-cost method for visible fluorescence imaging.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Dynamic X-ray diffraction sampling for protein crystal positioning.Journal of synchrotron radiation · 2017Article
- Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging.Journal of synchrotron radiation · 2016Article
- A visible-light-excited fluorescence method for imaging protein crystals without added dyes.Journal of applied crystallography · 2016Article
- The detection and subsequent volume optimization of biological nanocrystals.Structural dynamics (Melville, N.Y.) · 2015Article
- Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals.Acta crystallographica. Section D, Biological crystallography · 2015Article
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3 authors.
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Abstract
Two-photon excited ultraviolet fluorescence (TPE-UVF) microscopy is explored for sensitive protein-crystal detection as a complement to second-order nonlinear optical imaging of chiral crystals (SONICC). Like conventional ultraviolet fluorescence (UVF), TPE-UVF generates image contrast based on the intrinsic fluorescence of aromatic residues, generally producing higher fluorescence emission within crystals than the mother liquor by nature of the higher local protein concentration. However, TPE-UVF has several advantages over conventional UVF, including (i) insensitivity to optical scattering, allowing imaging in turbid matrices, (ii) direct compatibility with conventional optical plates and windows by using visible light for excitation, (iii) elimination of potentially damaging out-of-plane UV excitation, (iv) improved signal to noise through background reduction from out-of-plane excitation and (v) relatively simple integration into instrumentation developed for SONICC.
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