ArticleProceedings of the National Academy of Sciences of the United States of America2011
piggyBac transposon remobilization and enhancer detection in Anopheles mosquitoes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- ImprovedInternational journal of molecular sciences · 2023Article
- Transient knockdown of Anopheles stephensi LRIM1 using RNAi increases Plasmodium falciparum sporozoite salivary gland infections.Malaria journal · 2021Article
- Making gene drive biodegradable.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes.Frontiers in bioengineering and biotechnology · 2021Article
- Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.BMC genetics · 2020Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- AnG3 (Bethesda, Md.) · 2018Article
- The interplay between dose and immune system activation determines fungal infection outcome in the African malaria mosquito, Anopheles gambiae.Developmental and comparative immunology · 2018Article
- Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?International journal of environmental research and public health · 2017Review
- nanos-Driven expression of piggyBac transposase induces mobilization of a synthetic autonomous transposon in the malaria vector mosquito, Anopheles stephensi.Insect biochemistry and molecular biology · 2017Article
- Control of Mosquito-Borne Infectious Diseases: Sex and Gene Drive.Trends in parasitology · 2016Review
- An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori.Scientific reports · 2015Article
- Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.PloS one · 2015Article
- Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi.Genome biology · 2014Article
- Efficient ΦC31 integrase-mediated site-specific germline transformation of Anopheles gambiae.Nature protocols · 2014Article
- Identification of a novel strong and ubiquitous promoter/enhancer in the silkworm Bombyx mori.G3 (Bethesda, Md.) · 2014Article
- A nucleolus-predominant piggyBac transposase, NP-mPB, mediates elevated transposition efficiency in mammalian cells.PloS one · 2014Article
- Germline transformation of the spotted wing drosophilid, Drosophila suzukii, with a piggyBac transposon vector.Genetica · 2013Article
- piggyBac: A vehicle for integrative DNA transformation of parasitic nematodes.Mobile genetic elements · 2013Article
- Article
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5 authors.
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Abstract
Technical advances in mosquito biology are enabling the development of new approaches to vector control. Absent are powerful forward-genetics technologies, such as enhancer and gene traps, that permit determination of gene functions from the phenotypes arising from transposon insertion mutations. We show that the piggyBac transposon is highly active in the germline of the human malaria vector Anopheles stephensi. Up to 6% of the progeny from transgenic A. stephensi containing a single 6-kb piggyBac element with a marker gene expressing EGFP had the vector in new genomic locations when piggyBac transposase was provided in trans from a second integrated transgene. The active transposition of piggyBac resulted in the efficient detection of enhancers, with ~10% of the progeny with piggyBac in new locations with novel patterns of EGFP expression in third and fourth instar larvae and in adults. The availability of advanced transgenic capabilities such as efficient transposon-based forward-genetics technologies for Anopheles mosquitoes not only will accelerate our understanding of mosquito functional genomics and the development of novel vector and disease transmission control strategies, but also will enable studies by evolutionary developmental biologists, virologists, and parasitologists.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.