ArticleMolecular and cellular biology2011
Requirement of rRNA methylation for 80S ribosome assembly on a cohort of cellular internal ribosome entry sites.
Article in Molecular and cellular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 71 citations in OpenAlex.
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- 18S rRNA methyltransferases DIMT1 and BUD23 drive intergenerational hormesis.Molecular cell · 2023Article
- FBL promotes cancer cell resistance to DNA damage and BRCA1 transcription via YBX1.EMBO reports · 2023Article
- Regulation of translation by ribosomal RNA pseudouridylation.Science advances · 2023Article
- Current research on viral proteins that interact with fibrillarin.Molecular biology reports · 2023Review
- RNA methylation and cellular response to oxidative stress-promoting anticancer agents.Cell cycle (Georgetown, Tex.) · 2023Review
- The emerging importance of METTL5-mediated ribosomal RNA methylation.Experimental & molecular medicine · 2022Review
- Small but strong: Pivotal roles and potential applications of snoRNAs in hematopoietic malignancies.Frontiers in oncology · 2022Review
- Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing.Nature biotechnology · 2021Article
- Small nucleolar RNA is potential as a novel player in leukemogenesis and clinical application.Blood science (Baltimore, Md.) · 2021Review
- Regulation of Ribosome Function by RNA Modifications in Hematopoietic Development and Leukemia: It Is Not Only a Matter of mInternational journal of molecular sciences · 2021Review
- The non-coding epitranscriptome in cancer.Briefings in functional genomics · 2021Review
- Regulation of translation by methylation multiplicity of 18S rRNA.Cell reports · 2021Article
- Hypoxia re-programs 2'-O-Me modifications on ribosomal RNA.iScience · 2021Article
- RNA modifications and cancer.RNA biology · 2020Review
- The 18S rRNA mEMBO reports · 2020Article
- Transcriptome Analyses and Antioxidant Activity Profiling Reveal the Role of a Lignin-Derived Biostimulant Seed Treatment in Enhancing Heat Stress Tolerance in Soybean.Plants (Basel, Switzerland) · 2020Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Protein syntheses mediated by cellular and viral internal ribosome entry sites (IRESs) are believed to have many features in common. Distinct mechanisms for ribosome recruitment and preinitiation complex assembly between the two processes have not been identified thus far. Here we show that the methylation status of rRNA differentially influenced the mechanism of 80S complex formation on IRES elements from the cellular sodium-coupled neutral amino acid transporter 2 (SNAT2) versus the hepatitis C virus mRNA. Translation initiation involves the assembly of the 48S preinitiation complex, followed by joining of the 60S ribosomal subunit and formation of the 80S complex. Abrogation of rRNA methylation did not affect the 48S complex but resulted in impairment of 80S complex assembly on the cellular, but not the viral, IRESs tested. Impairment of 80S complex assembly on the amino acid transporter SNAT2 IRES was rescued by purified 60S subunits containing fully methylated rRNA. We found that rRNA methylation did not affect the activity of any of the viral IRESs tested but affected the activity of numerous cellular IRESs. This work reveals a novel mechanism operating on a cohort of cellular IRESs that involves rRNA methylation for proper 80S complex assembly and efficient translation initiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.