ArticleBMC veterinary research2011
Effectiveness of combination of Mini-and Microsatellite loci to sub-type Mycobacterium avium subsp. paratuberculosis Italian type C isolates.
Article in BMC veterinary research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- The Results After One Year of an Experimental Protocol Aimed at Reducing Paratuberculosis in an Intensive Dairy Herd.Animals : an open access journal from MDPI · 2025Article
- High Molecular Diversity ofInternational journal of molecular sciences · 2025Article
- Active surveillance of paratuberculosis in Alpine-dwelling red deer (Frontiers in veterinary science · 2024Article
- Goat Paratuberculosis: Experimental Model for the Evaluation of Mycobacterium Persistence in Raw Milk Cheese.Microorganisms · 2021Article
- Early Detection ofFrontiers in veterinary science · 2021Article
- Paratuberculosis in Captive Scimitar-Horned Oryxes (Animals : an open access journal from MDPI · 2020Article
- Spectroscopic Characterization of Bovine, Avian and Johnin Purified Protein Derivative (PPD) with High-Throughput Fourier Transform InfraRed-Based Method.Pathogens (Basel, Switzerland) · 2019Article
- Serological, culture and molecular survey of Mycobacterium avium paratuberculosis in a goat flock in Tuscany.Folia microbiologica · 2017Article
- Isolation, Identification, and Characterization of a New Highly Pathogenic Field Isolate ofFrontiers in veterinary science · 2017Article
- Typing of Mycobacterium avium subspecies paratuberculosis isolates from Newfoundland using fragment analysis.PloS one · 2015Article
- Genetic structure of Mycobacterium avium subsp. paratuberculosis population in cattle herds in Quebec as revealed by using a combination of multilocus genomic analyses.Journal of clinical microbiology · 2014Article
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Abstract
backgroundMycobacterium avium subsp. paratuberculosis (Map) is the etiological agent of paratuberculosis. The aim of our study was to combine Mini-and Microsatellite loci analysis in order to explore the effectiveness of this sub-typing method in a group of Map isolates. For this purpose, 84 Italian Type C Map isolates, each from a different cattle herd, were submitted to MIRU-Variable-Number Tandem-Repeats (VNTRs) typing and Short Sequence repeats (SSRs) sequencing. Moreover, the method was used to analyse the variability inside 10 herds (from three to 50 isolates per herd).
resultsThe molecular sub-typing, carried out using three SSR and 10 MIRU-VNTR loci, differentiated the 84 isolates into 33 clusters, reaching a Simpson's Discriminatory Index (SID) value of 0.952 (0.933 to 0.972, 95% confidence intervals). Among all considered loci, six (SSR2, MIRU2, SSR1, SSR8, VNTR3527 and VNTR1067) showed relevant allelic variability. Thirty-eight% of the isolates were clustered into four genotypes, differing from each other for the SSR2 locus. The other isolates, characterised by differences in two or more loci, were spread among the rest of the clusters. The intra-herd analysis revealed more than one genotype in most herds with a similar distribution of clusters.
conclusionsOur results revealed the advantage of using both Mini-and Microsatellite approaches for successfully discriminating among Map Type C isolates from the same geographic area, host species and herd. These data suggest that the combination of loci here proposed could be a useful molecular tool for regional epidemiological studies.
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