ArticleBiochemistry2011
Post-translational modifications and lipid binding profile of insect cell-expressed full-length mammalian synaptotagmin 1.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- The Core Complex of the CaJournal of molecular biology · 2023Review
- Calcium Sensors of Neurotransmitter Release.Advances in neurobiology · 2023Article
- Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy.Molecular biomedicine · 2022Review
- Lead exposure induces nitrative stress and disrupts ribbon synapses in the cochlea.Frontiers in molecular neuroscience · 2022Article
- Gangliosides interact with synaptotagmin to form the high-affinity receptor complex for botulinum neurotoxin B.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular Mechanisms of Fast Neurotransmitter Release.Annual review of biophysics · 2018Review
- Review
- Structural Impact of Phosphorylation and Dielectric Constant Variation on Synaptotagmin's IDR.Biophysical journal · 2018Article
- Article
- Gasotransmitter Heterocellular Signaling.Antioxidants & redox signaling · 2017Article
- Pull-down combined with proteomic strategy reveals functional diversity of synaptotagmin I.PeerJ · 2017Article
- PtdInsPeLife · 2016Article
- Nitric Oxide-Mediated Posttranslational Modifications: Impacts at the Synapse.Oxidative medicine and cellular longevity · 2016Review
- Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae.AMB Express · 2015Article
- Synaptotagmin-1 binds to PIP(2)-containing membrane but not to SNAREs at physiological ionic strength.Nature structural & molecular biology · 2015Article
- Article
- The juxtamembrane linker of full-length synaptotagmin 1 controls oligomerization and calcium-dependent membrane binding.The Journal of biological chemistry · 2014Article
- Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment.Nature structural & molecular biology · 2013Article
- Ras GTPase activating (RasGAP) activity of the dual specificity GAP protein Rasal requires colocalization and C2 domain binding to lipid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Hydrophobic contributions to the membrane docking of synaptotagmin 7 C2A domain: mechanistic contrast between isoforms 1 and 7.Biochemistry · 2012Article
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Authors and funding
6 authors at 5 institutions in 1 country.
Funding
Abstract
Synaptotagmin 1 (Syt1) is a Ca(2+) sensor for SNARE-mediated, Ca(2+)-triggered synaptic vesicle fusion in neurons. It is composed of luminal, transmembrane, linker, and two Ca(2+)-binding (C2) domains. Here we describe expression and purification of full-length mammalian Syt1 in insect cells along with an extensive biochemical characterization of the purified protein. The expressed and purified protein is properly folded and has increased α-helical content compared to the C2AB fragment alone. Post-translational modifications of Syt1 were analyzed by mass spectrometry, revealing the same modifications of Syt1 that were previously described for Syt1 purified from brain extract or mammalian cell lines, along with a novel modification of Syt1, tyrosine nitration. A lipid binding screen with both full-length Syt1 and the C2AB fragments of Syt1 and Syt3 isoforms revealed new Syt1-lipid interactions. These results suggest a conserved lipid binding mechanism in which Ca(2+)-independent interactions are mediated via a lysine rich region of the C2B domain while Ca(2+)-dependent interactions are mediated via the Ca(2+)-binding loops.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.