ArticleEuropean biophysics journal : EBJ2011
Automated multidimensional single molecule fluorescence microscopy feature detection and tracking.
Article in European biophysics journal : EBJ, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Drug-resistant EGFR mutations promote lung cancer by stabilizing interfaces in ligand-free kinase-active EGFR oligomers.Nature communications · 2024Article
- A perspective of fluorescence microscopy for cellular structural biology with EGFR as witness.Journal of microscopy · 2023Article
- AR cooperates with SMAD4 to maintain skeletal muscle homeostasis.Acta neuropathologica · 2022Article
- upU-Net Approaches for Background Emission Removal in Fluorescence Microscopy.Journal of imaging · 2022Article
- Fluorescence Imaging of Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor Resistance in Non-Small Cell Lung Cancer.Cancers · 2022Review
- Quantifying the Organization and Dynamics of the Plant Plasma Membrane Across Scales Using Light Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Bayesian Inference: The Comprehensive Approach to Analyzing Single-Molecule Experiments.Annual review of biophysics · 2021Review
- A small molecule inhibitor of HER3: a proof-of-concept study.The Biochemical journal · 2020Article
- The cell wall regulates dynamics and size of plasma-membrane nanodomains inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Brief History of Single-Particle Tracking of the Epidermal Growth Factor Receptor.Methods and protocols · 2019Review
- A global sampler of single particle tracking solutions for single molecule microscopy.PloS one · 2019Article
- The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers.Nature communications · 2018Article
- Article
- Automated single particle detection and tracking for large microscopy datasets.Royal Society open science · 2016Article
- Image Restoration and Analysis of Influenza Virions Binding to Membrane Receptors Reveal Adhesion-Strengthening Kinetics.PloS one · 2016Article
- Effect of phosphorylation on EGFR dimer stability probed by single-molecule dynamics and FRET/FLIM.Biophysical journal · 2015Article
- Imaging single chiral nanoparticles in turbid media using circular-polarization optical coherence microscopy.Scientific reports · 2014Article
- Single-molecule detection and tracking in plants.Protoplasma · 2014Review
- Measuring EGFR separations on cells with ~10 nm resolution via fluorophore localization imaging with photobleaching.PloS one · 2013Article
- A stochastic model for electron multiplication charge-coupled devices--from theory to practice.PloS one · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Characterisation of multi-protein interactions in cellular networks can be achieved by optical microscopy using multidimensional single molecule fluorescence imaging. Proteins of different species, individually labelled with a single fluorophore, can be imaged as isolated spots (features) of different colour light in different channels, and their diffusive behaviour in cells directly measured through time. Challenges in data analysis have, however, thus far hindered its application in biology. A set of methods for the automated analysis of multidimensional single molecule microscopy data from cells is presented, incorporating Bayesian segmentation-based feature detection, image registration and particle tracking. Single molecules of different colours can be simultaneously detected in noisy, high background data with an arbitrary number of channels, acquired simultaneously or time-multiplexed, and then tracked through time. The resulting traces can be further analysed, for example to detect intensity steps, count discrete intensity levels, measure fluorescence resonance energy transfer (FRET) or changes in polarisation. Examples are shown illustrating the use of the algorithms in investigations of the epidermal growth factor receptor (EGFR) signalling network, a key target for cancer therapeutics, and with simulated data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.