ArticleParasitology research2012
Tandem repeat protein as potential diagnostic antigen for Trypanosoma evansi infection.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- Domestic dogs as reservoirs for African trypanosomiasis in Mambwe district, eastern Zambia.Scientific reports · 2024Article
- Comparative membrane proteomic analysis of Tritrichomonas foetus isolates.Scientific reports · 2024Article
- A review on the diagnosis of animal trypanosomoses.Parasites & vectors · 2022Review
- Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis.Scientific reports · 2022Article
- The increased presence of repetitive motifs in the KDDR-plus recombinant protein, a kinesin-derived antigen from Leishmania infantum, improves the diagnostic performance of serological tests for human and canine visceral leishmaniasis.PLoS neglected tropical diseases · 2021Article
- Nationwide serological surveillance of non-tsetse-transmitted horse trypanosomoses in Mongolia.Parasite epidemiology and control · 2020Article
- Equine trypanosomosis: enigmas and diagnostic challenges.Parasites & vectors · 2019Review
- Article
- The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia.Parasitology research · 2018Article
- High-Sensitivity Assays for Plasmodium falciparum Infection by Immuno-Polymerase Chain Reaction Detection of PfIDEh and PfLDH Antigens.The Journal of infectious diseases · 2017Article
- Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia.Parasites & vectors · 2016Article
- Important hemoprotozoan diseases of livestock: Challenges in current diagnostics and therapeutics: An update.Veterinary world · 2016Review
- A TeGM6-4r antigen-based immunochromatographic test (ICT) for animal trypanosomosis.Parasitology research · 2015Article
- Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa.The Journal of veterinary medical science · 2015Article
- Variant surface glycoproteins from Venezuelan trypanosome isolates are recognized by sera from animals infected with either Trypanosoma evansi or Trypanosoma vivax.Veterinary parasitology · 2015Article
- Identification and characterization of a Trypanosoma congolense 46 kDa protein as a candidate serodiagnostic antigen.The Journal of veterinary medical science · 2014Article
- Expression, immunolocalization and serodiagnostic value of Tc38630 protein from Trypanosoma congolense.Parasitology research · 2013Article
- Trypanosoma vivax GM6 antigen: a candidate antigen for diagnosis of African animal trypanosomosis in cattle.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypanosoma evansi infection (surra) causes significant losses in livestock production in tropical and sub-tropical areas. The current ELISA recommended by OIE for diagnosis of the disease is based on trypanosome lysate antigen. However, antigenic variation and unstable nature of cell lysate antigen make it difficult to standardize the assay. Thus, there are needs to develop recombinant antigen-based ELISA that improve stability, sensitivity, and specificity of the test. Since tandem repeat (TR) proteins of trypanosomatid parasites generally possess high antigenicity, they have been considered to be the promising antigens for trypanosomosis and leishmaniosis. In this study, IgG responses against 14 recombinant TR proteins of trypanosomes were examined by ELISA. Serum samples were obtained from three water buffaloes experimentally infected with T. evansi. Since Trypanosoma congolense GM6 (TcoGM6) elicited highest IgG responses to all water buffaloes, we further bioinformatically and molecular biologically identified Trypanosoma brucei brucei GM6 (TbbGM6) and T. evansi GM6 (TeGM6) TR genes, respectively. As expected, predicted amino acid sequences of TbbGM6 and TeGM6 were identical while the nucleic acid sequence homology between TbbGM6 and TcoGM6 was 63.8%. All buffaloes became clearly positive in recombinant TbbGM6 (rTbbGM6)-based ELISA at 48 days post-infection, suggesting that rTbbGM6 is usable as a serodiagnostic antigen for chronic T. evansi infection.
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