ArticleActa histochemica et cytochemica2011
Immunohistochemical Analysis of Histone H3 Modifications in Germ Cells during Mouse Spermatogenesis.
Article in Acta histochemica et cytochemica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 146 citations in OpenAlex.
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- Unmasking the Epigenome: Insights into Testicular Cell Dynamics and Reproductive Function.International journal of molecular sciences · 2025Review
- Epigenetics of nonobstructive azoospermia.Asian journal of andrology · 2025Review
- Reflections on the Principles of Fixation in Histochemistry and Cytochemistry: With a Special Focus on Immunohistochemistry andActa histochemica et cytochemica · 2025Review
- Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp.Scientific reports · 2025Article
- Development of CAR-T cell therapy for NF1/SWN-related nerve sheath tumor treatment.Acta neuropathologica communications · 2025Article
- Emerging evidence that the mammalian sperm epigenome serves as a template for embryo development.Nature communications · 2023Review
- A gene-encoded FRET fluorescent sensor designed for detecting asymmetric dimethylation levels in vitro and in living cells.Analytical and bioanalytical chemistry · 2023Article
- Epigenetic markers in the embryonal germ cell development and spermatogenesis.Basic and clinical andrology · 2023Review
- Transcriptome profiling of histone writers/erasers enzymes across spermatogenesis, mature sperm and pre-cleavage embryo: Implications in paternal epigenome transitions and inheritance mechanisms.Frontiers in cell and developmental biology · 2023Article
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- MRNIP interacts with sex body chromatin to support meiotic progression, spermatogenesis, and male fertility in mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Cloning and Expression Analysis ofAnimals : an open access journal from MDPI · 2022Article
- Male triploid oysters of Crassostrea gigas exhibit defects in mitosis and meiosis during early spermatogenesis.FEBS open bio · 2022Article
- Chromosome-level genome assembly of grass carp (Ctenopharyngodon idella) provides insights into its genome evolution.BMC genomics · 2022Article
- Microenvironment for spermatogenesis and sperm maturation.Histochemistry and cell biology · 2022Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histone modification has been implicated in the regulation of mammalian spermatogenesis. However, the association of differently modified histone H3 with a specific stage of germ cells during spermatogenesis is not fully understood. In this study, we examined the localization of variously modified histone H3 in paraffin-embedded sections of adult mouse testis immunohistochemically, focusing on acetylation at lysine 9 (H3K9ac), lysine 18 (H3K18ac), and lysine 23 (H3K23ac); tri-methylation at lysine 4 (H3K4me3) and lysine 27 (H3K27me3); and phosphorylation at serine 10 (H3S10phos). As a result, we found that there was a significant fluctuation in the modifications; in spermatogonia, the stainings for H3K9ac, H3K18ac, and H3K23ac were strong while that for H3K4me3 was weak. In spermatocytes, the stainings for H3K9ac, H3K18ac, H3K23ac, and H3K4me3 were reduced in the preleptotene to pachytene stage, but in diplotene stage the stainings for H3K18ac, H3K23ac, and H3K4me3 seemed to become intense again. The staining for H3K27me3 was nearly constant throughout these stages. In the ensuing spermiogenesis, a dramatic acetylation and methylation of histone H3 was found in the early elongated spermatids and then almost all signals disappeared in the late elongated spermatids, in parallel with the replacement from histones to protamines. In addition, we confirmed that the staining of histone H3S10phos was exclusively associated with mitotic and meiotic cell division. Based upon the above results, we indicated that the modification pattern of histone H3 is subject to dynamic change and specific to a certain stage of germ cell differentiation during mouse spermatogenesis.
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