ArticleNature cell biology2011
Apicobasal domain identities of expanding tubular membranes depend on glycosphingolipid biosynthesis.
Article in Nature cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
72 citing papers in PubMed, 1 synthesis or guideline pooled it, 139 citations in OpenAlex.
- Trends in Glucocerebrosides Research: A Systematic Review.Frontiers in physiology · 2020Pooled it
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- SNX-3 confers lysosomal fusion-competence to sustain basal autophagy.Cellular and molecular life sciences : CMLS · 2026Article
- Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems.Cell reports · 2025Article
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- LET-767 determines lipid droplet protein targeting and lipid homeostasis.The Journal of cell biology · 2024Article
- Glycolipids in Parkinson's disease: beyond neuronal function.FEBS open bio · 2023Review
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- The biosynthetic-secretory pathway, supplemented by recycling routes, determines epithelial membrane polarity.Science advances · 2023Article
- Branched-chain actin dynamics polarizes vesicle trajectories and partitions apicobasal epithelial membrane domains.Science advances · 2023Article
- The impact ofmicroPublication biology · 2023Article
- Defining the glucosylceramide population ofFrontiers in physiology · 2023Review
- Proteotoxic stress disrupts epithelial integrity by inducing MTOR sequestration and autophagy overactivation.Autophagy · 2023Article
- Apical-basal polarity and the control of epithelial form and function.Nature reviews. Molecular cell biology · 2022Review
- Self-organization of apical membrane protein sorting in epithelial cells.The FEBS journal · 2022Article
- Membrane Lipids in Epithelial Polarity: Sorting out the PIPs.Frontiers in cell and developmental biology · 2022Review
- Regulation and functions of membrane lipids: Insights fromBBA advances · 2022Review
- Asymmetric organelle positioning during epithelial polarization of C. elegans intestinal cells.Developmental biology · 2022Article
- mmBCFA C17iso ensures endoplasmic reticulum integrity for lipid droplet growth.The Journal of cell biology · 2021Article
- Saturated very long chain fatty acid configures glycosphingolipid for lysosome homeostasis in long-lived C. elegans.Nature communications · 2021Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Metazoan internal organs are assembled from polarized tubular epithelia that must set aside an apical membrane domain as a lumenal surface. In a global Caenorhabditis elegans tubulogenesis screen, interference with several distinct fatty-acid-biosynthetic enzymes transformed a contiguous central intestinal lumen into multiple ectopic lumens. We show that multiple-lumen formation is caused by apicobasal polarity conversion, and demonstrate that in situ modulation of lipid biosynthesis is sufficient to reversibly switch apical domain identities on growing membranes of single post-mitotic cells, shifting lumen positions. Follow-on targeted lipid-biosynthesis pathway screens and functional genetic assays were designed to identify a putative single causative lipid species. They demonstrate that fatty-acid biosynthesis affects polarity through sphingolipid synthesis, and reveal ceramide glucosyltransferases (CGTs) as end-point biosynthetic enzymes in this pathway. Our findings identify glycosphingolipids, CGT products and obligate membrane lipids, as critical determinants of in vivo polarity and indicate that they sort new components to the expanding apical membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.