ArticleEukaryotic cell2011
Branch point identification and sequence requirements for intron splicing in Plasmodium falciparum.
Article in Eukaryotic cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Deletion of splicing factor Cdc5 in Toxoplasma disrupts transcriptome integrity, induces abortive bradyzoite formation, and prevents acute infection in mice.Nature communications · 2025Article
- Global dysregulation of circular RNAs in frontal cortex and whole blood from DM1 and DM2.Human genetics · 2025Article
- A central CRMP complex essential for invasion in Toxoplasma gondii.PLoS biology · 2023Article
- Article
- The Plasmodium falciparum rhoptry bulb protein RAMA plays an essential role in rhoptry neck morphogenesis and host red blood cell invasion.PLoS pathogens · 2019Article
- Full-Length Transcriptome Sequencing and the Discovery of New Transcripts in the Unfertilized Eggs of Zebrafish (G3 (Bethesda, Md.) · 2019Article
- Diverse target gene modifications in Plasmodium falciparum using Bxb1 integrase and an intronic attB.Parasites & vectors · 2018Review
- 50/50 Expressional Odds of Retention Signifies the Distinction between Retained Introns and Constitutively Spliced Introns inFrontiers in plant science · 2017Article
- An ultrasensitive NanoLuc-based luminescence system for monitoring Plasmodium berghei throughout its life cycle.Malaria journal · 2016Article
- A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum.Scientific reports · 2016Article
- A Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like Format.PloS one · 2015Article
- Enlightening the malaria parasite life cycle: bioluminescent Plasmodium in fundamental and applied research.Frontiers in microbiology · 2015Review
- Transcript maturation in apicomplexan parasites.Current opinion in microbiology · 2014Review
- The role played by alternative splicing in antigenic variability in human endo-parasites.Parasites & vectors · 2014Review
- The case for a rational genome-based vaccine against malaria.Frontiers in microbiology · 2014Review
- Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Splicing of mRNA is an ancient and evolutionarily conserved process in eukaryotic organisms, but intron-exon structures vary. Plasmodium falciparum has an extreme AT nucleotide bias (>80%), providing a unique opportunity to investigate how evolutionary forces have acted on intron structures. In this study, we developed an in vivo luciferase reporter splicing assay and employed it in combination with lariat isolation and sequencing to characterize 5' and 3' splicing requirements and experimentally determine the intron branch point in P. falciparum. This analysis indicates that P. falciparum mRNAs have canonical 5' and 3' splice sites. However, the 5' consensus motif is weakly conserved and tolerates nucleotide substitution, including the fifth nucleotide in the intron, which is more typically a G nucleotide in most eukaryotes. In comparison, the 3' splice site has a strong eukaryotic consensus sequence and adjacent polypyrimidine tract. In four different P. falciparum pre-mRNAs, multiple branch points per intron were detected, with some at U instead of the typical A residue. A weak branch point consensus was detected among 18 identified branch points. This analysis indicates that P. falciparum retains many consensus eukaryotic splice site features, despite having an extreme codon bias, and possesses flexibility in branch point nucleophilic attack.
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Registered trials
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