ArticleJournal of bacteriology2011
Deciphering morphological determinants of the helix-shaped Leptospira.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Leptospira interrogans biofilm transcriptome highlights adaption to starvation and general stress while maintaining virulence.NPJ biofilms and microbiomes · 2024Article
- A large screen identifies beta-lactam antibiotics which can be repurposed to target the syphilis agent.npj antimicrobials and resistance · 2023Article
- Host and Species-Specificities of Pattern Recognition Receptors Upon Infection WithFrontiers in cellular and infection microbiology · 2022Review
- A CRISPR interference platform for selective downregulation of gene expression inApplied and environmental microbiology · 2021Article
- A bacterial cytolinker couples positioning of magnetic organelles to cell shape control.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies.Scientific reports · 2020Article
- Leptospirosis: a neglected tropical zoonotic infection of public health importance-an updated review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Review
- Spirochete Flagella and Motility.Biomolecules · 2020Review
- The Role ofFrontiers in cellular and infection microbiology · 2019Article
- The mechanism of two-phase motility in the spirocheteScience advances · 2018Article
- A widely conserved bacterial cytoskeletal component influences unique helical shape and motility of the spirochete Leptospira biflexa.Molecular microbiology · 2018Article
- PathogenicOpen veterinary journal · 2018Review
- Leptospiral flagellar sheath protein FcpA interacts with FlaA2 and FlaB1 in Leptospira biflexa.PloS one · 2018Article
- LipL21 lipoprotein binding to peptidoglycan enables Leptospira interrogans to escape NOD1 and NOD2 recognition.PLoS pathogens · 2017Article
- Virulence of the zoonotic agent of leptospirosis: still terra incognita?Nature reviews. Microbiology · 2017Review
- Leptospiral outer membrane protein LipL32 induces inflammation and kidney injury in zebrafish larvae.Scientific reports · 2016Article
- Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments.Microbiology and molecular biology reviews : MMBR · 2016Review
- Article
- Leptospira interrogans lpxD Homologue Is Required for Thermal Acclimatization and Virulence.Infection and immunity · 2015Article
- Control of Gene Expression in Leptospira spp. by Transcription Activator-Like Effectors Demonstrates a Potential Role for LigA and LigB in Leptospira interrogans Virulence.Applied and environmental microbiology · 2015Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape. Defining the mechanisms by which these bacteria generate and maintain this atypical morphology should greatly enhance our understanding of the fundamental physiology of these pathogens. In this study, we showed that peptidoglycan sacculi from Leptospira spp. retain the helical shape of intact cells. Interestingly, the distribution of muropeptides was different from that in the Escherichia coli model, indicating that specific enzymes might be active on the peptidoglycan macromolecule. We could alter the shape of Leptospira biflexa with the broad-spectrum β-lactam antibiotic penicillin G and with amdinocillin and aztreonam, which are β-lactams that preferentially target penicillin-binding protein 2 (PBP2) and PBP3, respectively, in some species. Although genetic manipulations of Leptospira spp. are scarce, we were able to obtain mutants with alterations in genes encoding PBPs, including PBP3. Loss of this protein resulted in cell elongation. We also generated an L. biflexa strain that conditionally expresses MreB. Loss of the MreB function was correlated with morphological abnormalities such as a localized increased diameter and heterogeneous length. A prolonged depletion of MreB resulted in cell lysis, suggesting that this protein is essential. These findings indicate that important aspects of leptospiral cell morphology are determined by the cytoskeleton and the murein layer, thus providing a starting point for a better understanding of the morphogenesis in these atypical bacteria.
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