ArticleJournal of bacteriology2011
FadD is required for utilization of endogenous fatty acids released from membrane lipids.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 74 citations in OpenAlex.
- Defining the Enterococcus faecalis Fatty Acid Kinase System of Exogeneous Fatty Acid Utilization.Molecular microbiology · 2025Article
- Identification of a Sinorhizobium meliloti YbgC-like thioesterase that contributes to the production of the infochemical 2-tridecanone.The Biochemical journal · 2025Article
- Diet-Microbiota-Host Interactions in Regulation of Cardiometabolic Homeostasis: Emerging Mechanisms and Therapeutic Potential.Microbiota and host · 2025Article
- Bacterial acquisition of host fatty acids has far-reaching implications on virulence.Microbiology and molecular biology reviews : MMBR · 2024Review
- The puzzle of two tandem acyl-CoA ligases ofApplied and environmental microbiology · 2024Article
- Exploring Fatty Acid β-Oxidation Pathways in Bacteria: From General Mechanisms to DSF Signaling and Pathogenicity in Xanthomonas.Current microbiology · 2024Review
- Exploring engineering strategies that enhance de novo production of exotic cyclopropane fatty acids in Saccharomyces cerevisiae.Biotechnology journal · 2024Article
- A periplasmic phospholipase that maintains outer membrane lipid asymmetry inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Degradation of Exogenous Fatty Acids inBiomolecules · 2022Review
- Static Magnetic Field Inhibits Growth ofCells · 2022Article
- Primordial mimicry induces morphological change in Escherichia coli.Communications biology · 2022Article
- Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site.Microbiology (Reading, England) · 2021Article
- A Futile Metabolic Cycle of Fatty Acyl-CoA Hydrolysis and Resynthesis inApplied and environmental microbiology · 2021Article
- Role ofMicroorganisms · 2020Article
- ThemBio · 2018Article
- Comparative study on nutrient depletion-induced lipidome adaptations in Staphylococcus haemolyticus and Staphylococcus epidermidis.Scientific reports · 2018Article
- Dietary Fatty Acids Control the Species of N-Acyl-Phosphatidylethanolamines Synthesized by Therapeutically Modified Bacteria in the Intestinal Tract.ACS infectious diseases · 2018Article
- Engineering the fatty acid synthesis pathway inBiotechnology for biofuels · 2018Article
- A Rhodococcal Transcriptional Regulatory Mechanism Detects the Common Lactone Ring of AHL Quorum-Sensing Signals and Triggers the Quorum-Quenching Response.Frontiers in microbiology · 2018Article
- A genome-wide screen inThe Journal of biological chemistry · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
FadD is an acyl coenzyme A (CoA) synthetase responsible for the activation of exogenous long-chain fatty acids (LCFA) into acyl-CoAs. Mutation of fadD in the symbiotic nitrogen-fixing bacterium Sinorhizobium meliloti promotes swarming motility and leads to defects in nodulation of alfalfa plants. In this study, we found that S. meliloti fadD mutants accumulated a mixture of free fatty acids during the stationary phase of growth. The composition of the free fatty acid pool and the results obtained after specific labeling of esterified fatty acids with a Δ5-desaturase (Δ5-Des) were in agreement with membrane phospholipids being the origin of the released fatty acids. Escherichia coli fadD mutants also accumulated free fatty acids released from membrane lipids in the stationary phase. This phenomenon did not occur in a mutant of E. coli with a deficient FadL fatty acid transporter, suggesting that the accumulation of fatty acids in fadD mutants occurs inside the cell. Our results indicate that, besides the activation of exogenous LCFA, in bacteria FadD plays a major role in the activation of endogenous fatty acids released from membrane lipids. Furthermore, expression analysis performed with S. meliloti revealed that a functional FadD is required for the upregulation of genes involved in fatty acid degradation and suggested that in the wild-type strain, the fatty acids released from membrane lipids are degraded by β-oxidation in the stationary phase of growth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.