ArticleApplied and environmental microbiology2011
Engineering Escherichia coli for biodiesel production utilizing a bacterial fatty acid methyltransferase.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 108 citations in OpenAlex.
- PMTPred: machine-learning-based prediction of protein methyltransferases using the composition of k-spaced amino acid pairs.Molecular diversity · 2024Article
- Saccharomyces cerevisiae cellular engineering for the production of FAME biodiesel.AMB Express · 2024Article
- Isotope-labeling in situ derivatization and HS-SPME arrow GC-MS/MS for simultaneous determination of fatty acids and fatty acid methyl esters in aqueous matrices.Analytical and bioanalytical chemistry · 2023Article
- A large conserved family of small-molecule carboxyl methyltransferases identified from microorganisms.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microbial pathways for advanced biofuel production.Biochemical Society transactions · 2022Review
- Metabolic engineering strategies to produce medium-chain oleochemicals via acyl-ACP:CoA transacylase activity.Nature communications · 2022Article
- Escherichia coli coculture for de novo production of esters derived of methyl-branched alcohols and multi-methyl branched fatty acids.Microbial cell factories · 2022Article
- The Role of Metabolic Engineering Technologies for the Production of Fatty Acids in Yeast.Biology · 2021Review
- Review
- Enhanced Production of Fatty Acid Ethyl Ester with EngineeredMicroorganisms · 2019Article
- Durable, acid-resistant copolymers from industrial by-product sulfur and microbially-produced tyrosine.RSC advances · 2019Article
- Establishing synthesis pathway-host compatibility via enzyme solubility.Biotechnology and bioengineering · 2019Article
- Metabolic Engineering for Enhanced Medium Chain Omega Hydroxy Fatty Acid Production inFrontiers in microbiology · 2018Article
- Microbial synthesis of medium-chain chemicals from renewables.Nature biotechnology · 2017Review
- Efficient production of free fatty acids from ionic liquid-based acid- or enzyme-catalyzed bamboo hydrolysate.Journal of industrial microbiology & biotechnology · 2017Article
- Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing.Journal of industrial microbiology & biotechnology · 2017Article
- Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals.Frontiers in microbiology · 2017Review
- Integrating Cellular and Bioprocess Engineering in the Non-Conventional YeastFrontiers in bioengineering and biotechnology · 2017Review
- Escherichia coli as a fatty acid and biodiesel factory: current challenges and future directions.Environmental science and pollution research international · 2016Review
- Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway.Microbial cell factories · 2016Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The production of low-cost biofuels in engineered microorganisms is of great interest due to the continual increase in the world's energy demands. Biodiesel is a renewable fuel that can potentially be produced in microbes cost-effectively. Fatty acid methyl esters (FAMEs) are a common component of biodiesel and can be synthesized from either triacylglycerol or free fatty acids (FFAs). Here we report the identification of a novel bacterial fatty acid methyltransferase (FAMT) that catalyzes the formation of FAMEs and 3-hydroxyl fatty acid methyl esters (3-OH-FAMEs) from the respective free acids and S-adenosylmethionine (AdoMet). FAMT exhibits a higher specificity toward 3-hydroxy free fatty acids (3-OH-FFAs) than FFAs, synthesizing 3-hydroxy fatty acid methyl esters (3-OH-FAMEs) in vivo. We have also identified bacterial members of the fatty acyl-acyl carrier protein (ACP) thioesterase (FAT) enzyme family with distinct acyl chain specificities. These bacterial FATs exhibit increased specificity toward 3-hydroxyacyl-ACP, generating 3-OH-FFAs, which can subsequently be utilized by FAMTs to produce 3-OH-FAMEs. PhaG (3-hydroxyacyl ACP:coenzyme A [CoA] transacylase) constitutes an alternative route to 3-OH-FFA synthesis; the coexpression of PhaG with FAMT led to the highest level of accumulation of 3-OH-FAMEs and FAMEs. The availability of AdoMet, the second substrate for FAMT, is an important factor regulating the amount of methyl esters produced by bacterial cells. Our results indicate that the deletion of the global methionine regulator metJ and the overexpression of methionine adenosyltransferase result in increased methyl ester synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.