ArticleMethods (San Diego, Calif.)2011
A rapid and robust method for selective isotope labeling of proteins.
Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 74 citations in OpenAlex.
- Optimized histidine-specific isotopic labeling in proteins overproduced in Escherichia coli.Protein science : a publication of the Protein Society · 2026Article
- Conformation-selective detection of residues in solid proteins under magic-angle-spinning.Journal of biomolecular NMR · 2026Article
- Selective isotope labeling probes the chemical capacity and reaction mechanism of a heterobimetallic Mn/Fe protein.Journal of inorganic biochemistry · 2025Article
- Electrochemical Synergistic Ni/Co-Catalyzed Carbonylative Cross-Electrophile Coupling of Aryl and Alkyl Halides with CO.JACS Au · 2025Article
- A practical guide for the preparation of C1-labeled α-amino acids using aldehyde catalysis with isotopically labeled CONature protocols · 2024Article
- Origin of the multi-phasic quenching dynamics in the BLUF domains across the species.Nature communications · 2024Article
- E. coli "Stablelabel" S30 lysate for optimized cell-free NMR sample preparation.Journal of biomolecular NMR · 2023Article
- Aldehyde-catalysed carboxylate exchange in α-amino acids with isotopically labelled CONature chemistry · 2022Article
- Improving the Robustness of Engineered Bacteria to Nutrient Stress Using Programmed Proteolysis.ACS synthetic biology · 2022Article
- Extensively sparseJournal of biomolecular NMR · 2021Article
- Asparaginyl endopeptidases: enzymology, applications and limitations.Organic & biomolecular chemistry · 2021Review
- Capturing Peptide-GPCR Interactions and Their Dynamics.Molecules (Basel, Switzerland) · 2020Review
- Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs.Journal of biomolecular NMR · 2020Article
- Late metabolic precursors for selective aromatic residue labeling.Journal of biomolecular NMR · 2018Review
- Decarboxylation involving a ferryl, propionate, and a tyrosyl group in a radical relay yields hemeThe Journal of biological chemistry · 2018Article
- Application of Solution NMR to Structural Studies on α-Helical Integral Membrane Proteins.Molecules (Basel, Switzerland) · 2017Review
- X-ray transparent microfluidic chips for high-throughput screening and optimization ofBiomicrofluidics · 2017Article
- Trifluoroselenomethionine: A New Unnatural Amino Acid.Chembiochem : a European journal of chemical biology · 2016Article
- Highly efficient residue-selective labeling with isotope-labeled Ile, Leu, and Val using a new auxotrophic E. coli strain.Journal of biomolecular NMR · 2016Article
- In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.Biophysical journal · 2015Review
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Amino-acid selective isotope labeling of proteins offers numerous advantages in mechanistic studies by revealing structural and functional information unattainable from a crystallographic approach. However, efficient labeling of proteins with selected amino acids necessitates auxotrophic hosts, which are often not available. We have constructed a set of auxotrophs in a commonly used Escherichia coli expression strain C43(DE3), a derivative of E. coli BL21(DE3), which can be used for isotopic labeling of individual amino acids or sets of amino acids. These strains have general applicability to either soluble or membrane proteins that can be expressed in E. coli. We present examples in which proteins are selectively labeled with (13)C- and (15)N-amino acids and studied using magic-angle spinning solid-state NMR and pulsed EPR, demonstrating the utility of these strains for biophysical characterization of membrane proteins, radical-generating enzymes and metalloproteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.