ArticleMethods in cell biology2011
Generating conditional mutations in zebrafish using gene-trap mutagenesis.
Article in Methods in cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- zMADM (zebrafish mosaic analysis with double markers) for single-cell gene knockout and dual-lineage tracing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fishing forward and reverse: Advances in zebrafish phenomics.Mechanisms of development · 2018Review
- Conditional gene-trap mutagenesis in zebrafish.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Chicken β-globin insulators fail to shield the nkx2.5 promoter from integration site effects in zebrafish.Molecular genetics and genomics : MGG · 2013Article
- Novel strategies for gene trapping and insertional mutagenesis mediated byMobile genetic elements · 2013Article
- Article
- Regulation of zebrafish sleep and arousal states: current and prospective approaches.Frontiers in neural circuits · 2013Review
- Conditional control of gene function by an invertible gene trap in zebrafish.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
While several mutagenesis methods have been successfully applied in zebrafish, these mutations do not allow tissue- or temporal-specific functional analysis. We have developed a strategy that will allow tissue- or temporal-specific disruption of genes in zebrafish. This strategy combines gene-trap mutagenesis and FlEx modules containing target sites for site-specific recombinases. The gene-trap cassette is highly mutagenic in one orientation and nonmutagenic in the opposite orientation, with different fluorescent proteins as indicators of the orientation. The inclusion of the FlEx modules allows two rounds of stable inversion mediated by the Cre and Flp recombinases. This gene-trap cassette can be easily delivered via transposons. Through large-scale community-wide efforts, broad genome coverage can be obtained. This should allow investigation of cell/tissue-specific gene function of a wide range of genes.
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Registered trials
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