ArticleMolecular microbiology2011
RbsB (NTHI_0632) mediates quorum signal uptake in nontypeable Haemophilus influenzae strain 86-028NP.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 90 citations in OpenAlex.
- Two-Component Systems inVeterinary sciences · 2025Review
- Klebsiella pneumoniae AI-2 transporters mediate interspecies interactions and composition in a three-species biofilm community.NPJ biofilms and microbiomes · 2024Article
- DUF1127-containing protein and ProQ had opposite effects on biofilm formation in Vibrio alginolyticus.BMC microbiology · 2024Article
- A fungal metabolic regulator underlies infectious synergism during Candida albicans-Staphylococcus aureus intra-abdominal co-infection.Nature communications · 2024Article
- AI-2 quorum sensing-induced galactose metabolism activation in Streptococcus suis enhances capsular polysaccharide-associated virulence.Veterinary research · 2024Article
- Multimodal evaluation of drug antibacterial activity reveals cinnamaldehyde analog anti-biofilm effects againstBiofilm · 2024Article
- Transcriptome Reveals Regulation of Quorum Sensing ofFoods (Basel, Switzerland) · 2024Article
- Regulatory Mechanisms and Physiological Impacts of Quorum Sensing in Gram-Negative Bacteria.Infection and drug resistance · 2024Review
- Inhibition of Plasmid Conjugation inInternational journal of molecular sciences · 2023Article
- A 4-Hydroxybenzoic Acid-Mediated Signaling System Controls the Physiology and Virulence of Shigella sonnei.Microbiology spectrum · 2023Article
- Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies.Infection and drug resistance · 2023Review
- Article
- Comparative transcriptome analysis reveals that deletion of CheY influences gene expressions of ABC transports and metabolism in Haemophilus parasuis.Functional & integrative genomics · 2021Article
- Bacterial Quorum-Sensing Systems and Their Role in Intestinal Bacteria-Host Crosstalk.Frontiers in microbiology · 2021Review
- Quorum sensing across bacterial and viral domains.PLoS pathogens · 2021Article
- The Role of Non-TypeableFrontiers in cellular and infection microbiology · 2021Review
- NontypeableBiofilm · 2020Article
- Draft Genome Analysis ofJournal of genomics · 2020Article
- Review
- Quorum Sensing as Antivirulence Target in Cystic Fibrosis Pathogens.International journal of molecular sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Nontypeable Haemophilus influenzae (NTHI) is a respiratory commensal and opportunistic pathogen, which persists within biofilms on airway mucosal surfaces. For many species, biofilm formation is impacted by quorum signalling. Our prior work shows that production of autoinducer-2 (AI-2) promotes biofilm development and persistence for NTHI 86-028NP. NTHI 86-028NP encodes an ABC transporter annotated as a ribose transport system that includes a protein (RbsB) with similarity to the Escherichia coli LsrB and Aggregatibacter actinomycetemcomitans RbsB proteins that bind AI-2. In this study, inactivation of rbsB significantly reduced uptake of AI-2 and the AI-2 precursor dihydroxypentanedione (DPD) by NTHI 86-028NP. Moreover, DPD uptake was not competitively inhibited by ribose or other pentose sugars. Transcript levels of rbsB increased in response to DPD and as bacteria approached stationary-phase growth. The NTHI 86-028NP rbsB mutant also formed biofilms with significantly reduced thickness and total biomass and reduced surface phosphorylcholine, similar to a luxS mutant. Infection studies revealed that loss of rbsB impaired bacterial persistence in the chinchilla middle ear, similar to our previous results with luxS mutants. Based on these data, we conclude that in NTHI 86-028NP, RbsB is a LuxS/AI-2 regulated protein that is required for uptake of and response to AI-2.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.