ArticleBiochemistry2011
Variations in protein-flavin hydrogen bonding in a light, oxygen, voltage domain produce non-Arrhenius kinetics of adduct decay.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 57 citations in OpenAlex.
- Potent optogenetic regulation of gene expression in mammalian cells for bioproduction and basic research.Nucleic acids research · 2025Article
- Light-dependent flavin redox and adduct states control the conformation and DNA-binding activity of the transcription factor EL222.Nucleic acids research · 2025Article
- Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins inACS synthetic biology · 2024Article
- Optogenetic control of phosphate-responsive genes using single component fusion proteins inbioRxiv : the preprint server for biology · 2024Article
- A structural decryption of cryptochromes.Frontiers in chemistry · 2024Review
- Machine Learning-Assisted Engineering of Light, Oxygen, Voltage Photoreceptor Adduct Lifetime.JACS Au · 2023Article
- Genetically encoded non-canonical amino acids reveal asynchronous dark reversion of chromophore, backbone, and side-chains in EL222.Protein science : a publication of the Protein Society · 2023Article
- Optogenetic Amplification Circuits for Light-Induced Metabolic Control.ACS synthetic biology · 2021Article
- Light-induced local gene expression in primary chick cell culture system.Development, growth & differentiation · 2021Article
- Engineering of a Promoter Repressed by a Light-Regulated Transcription Factor inBiodesign research · 2021Article
- Toward Multiplexed Optogenetic Circuits.Frontiers in bioengineering and biotechnology · 2021Review
- Tunable Properties of Nature-InspiredMolecules (Basel, Switzerland) · 2020Article
- Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons.PloS one · 2020Article
- Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells.Scientific reports · 2018Article
- Novel Riboflavin-Inspired Conjugated Bio-Organic Semiconductors.Molecules (Basel, Switzerland) · 2018Article
- Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation.Nature communications · 2018Article
- A Native Threonine Coordinates Ordered Water to Tune Light-Oxygen-Voltage (LOV) Domain Photocycle Kinetics and Osmotic Stress Signaling in Trichoderma reesei ENVOY.The Journal of biological chemistry · 2016Article
- Short LOV Proteins in Methylocystis Reveal Insight into LOV Domain Photocycle Mechanisms.PloS one · 2015Article
- LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling.Frontiers in molecular biosciences · 2015Review
- Photo-sensitive degron variants for tuning protein stability by light.BMC systems biology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Light, oxygen, voltage (LOV) domains utilize a conserved blue light-dependent mechanism to control a diverse array of effector domains in biological and engineered proteins. Variations in the kinetics and efficiency of LOV photochemistry fine-tune various aspects of the photic response. Characterization of the kinetics of a key aspect of this photochemical mechanism in EL222, a blue light responsive DNA binding protein from Erythrobacter litoralis HTCC2594, reveals unique non-Arrhenius behavior in the rate of dark-state cleavage of the photochemically generated adduct. Sequence analysis and mutagenesis studies establish that this effect stems from a Gln to Ala mutation unique to EL222 and homologous proteins from marine bacteria. Kinetic and spectroscopic analyses reveal that hydrogen bonding interactions between the FMN N1, O2, and ribityl hydroxyls and the surrounding protein regulate photocycle kinetics and stabilize the LOV active site from temperature-induced alteration in local structure. Substitution of residues interacting with the N1-O2 locus modulates adduct stability, structural flexibility, and sequestration of the active site from bulk solvent without perturbation of light-activated DNA binding. Together, these variants link non-Arrhenius behavior to specific alteration of an H-bonding network, while affording tunability of photocycle kinetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.