ArticleThe Journal of biological chemistry2011
Novel role for mediator complex subunit Srb5/Med18 in termination of transcription.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 58 citations in OpenAlex.
- The Mediator complex: co-transcriptional regulation and beyond.RNA biology · 2026Review
- Genome-wide analysis of TFIIB's role in termination of transcription.Research square · 2024Article
- Dosage suppressors of gpn2ts mutants and functional insights into the role of Gpn2 in budding yeast.PloS one · 2024Article
- Mediator Complex of the Malaria ParasiteACS omega · 2022Article
- Increased unfolded protein responses caused by MED17 mutations.Neurogenetics · 2021Article
- MedProDB: A database of Mediator proteins.Computational and structural biotechnology journal · 2021Article
- Candida glabrata Med3 Plays a Role in Altering Cell Size and Budding Index To Coordinate Cell Growth.Applied and environmental microbiology · 2018Article
- Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae.Scientific reports · 2017Article
- Regulation of metabolism by the Mediator complex.Biophysics reports · 2016Article
- The Mediator subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination.The EMBO journal · 2015Article
- Gene looping facilitates TFIIH kinase-mediated termination of transcription.Scientific reports · 2015Article
- Proteomic analysis of the herpes simplex virus 1 virion protein 16 transactivator protein in infected cells.Proteomics · 2015Article
- Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants.Frontiers in plant science · 2015Review
- Upregulation of mediator MED23 in non-small-cell lung cancer promotes the growth, migration, and metastasis of cancer cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- A functional portrait of Med7 and the mediator complex in Candida albicans.PLoS genetics · 2014Article
- Liver Med23 ablation improves glucose and lipid metabolism through modulating FOXO1 activity.Cell research · 2014Article
- Kin28 regulates the transient association of Mediator with core promoters.Nature structural & molecular biology · 2014Article
- The head module of Mediator directs activation of preloaded RNAPII in vivo.Nucleic acids research · 2013Article
- Mechanisms of Mediator complex action in transcriptional activation.Cellular and molecular life sciences : CMLS · 2013Review
- Dismantling promoter-driven RNA polymerase II transcription complexes in vitro by the termination factor Rat1.The Journal of biological chemistry · 2013Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mediator complex functions at the recruitment as well as the post-recruitment steps of transcription. Here we provide evidence for a novel role of Mediator in termination of transcription. Mediator subunit Srb5/Med18 cross-links to the 5' and 3' ends of INO1 and CHA1. In srb5(-) cells, recruitment of TATA-binding protein (TBP) and transcription factor IIB (TFIIB) onto the promoter of these genes remained unaffected, but cross-linking of the cleavage-polyadenylation factors Rna15 and Pta1 toward the 3' end of genes was compromised. In these cells, RNA polymerase II accumulated near the 3' end of genes and beyond. Transcription run-on analysis confirmed a transcription readthrough phenotype in the absence of Srb5/Med18. These results strongly suggest that Mediator subunit Srb5/Med18 is required for proper termination of transcription of a subset of genes in budding yeast.
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