ArticleThe Journal of biological chemistry2011
Homeostasis of extracellular ATP in human erythrocytes.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- CR1-mediated ATP release by human red blood cells promotes CR1 clustering and modulates the immune transfer process.The Journal of biological chemistry · 2013Trial
- Erythrocytes as Active Regulators of Blood Flow: A Brief Overview.Advances in experimental medicine and biology · 2026Review
- Hypoxia induces robust ATP release from erythrocytes in ApoE-LDLR double-deficient mice.Frontiers in physiology · 2024Article
- Pharmacology of pannexin channels.Current opinion in pharmacology · 2023Review
- The Pleiotropic Role of Extracellular ATP in Myocardial Remodelling.Molecules (Basel, Switzerland) · 2023Review
- Npp1 prevents external tooth root resorption by regulation of cervical cementum integrity.Scientific reports · 2022Article
- A 3D-printed, multi-modal microfluidic device for measuring nitric oxide and ATP release from flowing red blood cells.Analytical methods : advancing methods and applications · 2022Article
- Mechanosensitive Pannexin 1 Activity Is Modulated by Stomatin in Human Red Blood Cells.International journal of molecular sciences · 2022Article
- The broad range di- and tri-nucleotide exchanger SLC35B1 displays asymmetrical affinities for ATP transport across the ER membrane.The Journal of biological chemistry · 2022Article
- Interactive Dynamics of Cell Volume and Cell Death in Human Erythrocytes Exposed to α-Hemolysin fromInternational journal of molecular sciences · 2022Article
- Red blood cells in type 1 diabetes and multiple sclerosis and technologies to measure their emerging roles.Journal of translational autoimmunity · 2022Review
- Sex-Specific Differences of Adenosine Triphosphate Levels in Red Blood Cells Isolated From ApoE/LDLR Double-Deficient Mice.Frontiers in physiology · 2022Article
- Evolution of Mechanical Stress (Archives of Razi Institute · 2021Article
- Hydrolysis of Extracellular ATP by Vascular Smooth Muscle Cells Transdifferentiated into Chondrocytes Generates PInternational journal of molecular sciences · 2021Article
- ATP-based therapy prevents vascular calcification and extends longevity in a mouse model of Hutchinson-Gilford progeria syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Red Blood Cell Deformability, Vasoactive Mediators, and Adhesion.Frontiers in physiology · 2019Review
- Review
- Induction of ATP Release, PPIX Transport, and Cholesterol Uptake by Human Red Blood Cells Using a New Family of TSPO Ligands.International journal of molecular sciences · 2018Article
- Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1.Scientific reports · 2018Article
- Erythrocyte's aging in microgravity highlights how environmental stimuli shape metabolism and morphology.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We explored the intra- and extracellular processes governing the kinetics of extracellular ATP (ATPe) in human erythrocytes stimulated with agents that increase cAMP. Using the luciferin-luciferase reaction in off-line luminometry we found both direct adenylyl cyclase activation by forskolin and indirect activation through β-adrenergic stimulation with isoproterenol-enhanced [ATP]e in a concentration-dependent manner. A mixture (3V) containing a combination of these agents and the phosphodiesterase inhibitor papaverine activated ATP release, leading to a 3-fold increase in [ATP]e, and caused increases in cAMP concentration (3-fold for forskolin + papaverine, and 10-fold for 3V). The pannexin 1 inhibitor carbenoxolone and a pannexin 1 blocking peptide ((10)Panx1) decreased [ATP]e by 75-84%. The residual efflux of ATP resulted from unavoidable mechanical perturbations stimulating a novel, carbenoxolone-insensitive pathway. In real-time luminometry experiments using soluble luciferase, addition of 3V led to an acute increase in [ATP]e to a constant value of ∼1 pmol × (10(6) cells)(-1). A similar treatment using a surface attached luciferase (proA-luc) triggered a rapid accumulation of surface ATP levels to a peak concentration of 2.4 pmol × (10(6) cells)(-1), followed by a slower exponential decay (t(½) = 3.7 min) to a constant value of 1.3 pmol × (10(6) cells)(-1). Both for soluble luciferase and proA-luc, ATP efflux was fully blocked by carbenoxolone, pointing to a 3V-induced mechanism of ATP release mediated by pannexin 1. Ecto-ATPase activity was extremely low (∼28 fmol × (10(6) cells min)(-1)), but nevertheless physiologically relevant considering the high density of erythrocytes in human blood.
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