ReviewInternational journal of hygiene and environmental health2011
Methods to detect infectious human enteric viruses in environmental water samples.
Review in International journal of hygiene and environmental health, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 2 syntheses or guidelines pooled it, 157 citations in OpenAlex.
- Occurrence of viruses in sewage sludge: A systematic review.The Science of the total environment · 2022Pooled it
- Risk management of viral infectious diseases in wastewater reclamation and reuse: Review.Environment international · 2016Pooled it
- Article
- Warming drinking water distribution systems in the context of climate change: a scoping review on health-related microbial and chemical water quality effects.Journal of water and health · 2025Article
- Enteroviruses in Water: Epidemiology, Detection and Inactivation.Environmental microbiology · 2025Review
- Common and Potential Emerging Foodborne Viruses: A Comprehensive Review.Life (Basel, Switzerland) · 2024Review
- Article
- Article
- Viral Metagenomics as a Tool to Track Sources of Fecal Contamination: A One Health Approach.Viruses · 2023Review
- Application of propidium monoazide quantitative PCR to discriminate of infectious African swine fever viruses.Frontiers in microbiology · 2023Article
- The Efficacy of Probiotics as Antiviral Agents for the Treatment of Rotavirus Gastrointestinal Infections in Children: An Updated Overview of Literature.Microorganisms · 2022Review
- Quantification of the Retention and Disassembly of Virus Particles by a PEI-Functionalized Microfiltration Membrane.ACS applied polymer materials · 2022Article
- Viral Eco-Genomic Tools: Development and Implementation for Aquatic Biomonitoring.International journal of environmental research and public health · 2022Review
- Coronaviruses and SARS-CoV-2 in sewerage and their removal: Step by step in wastewater treatment plants.Environmental research · 2022Review
- Optimizing Human Intestinal Enteroids for Environmental Monitoring of Human Norovirus.Food and environmental virology · 2021Article
- Capsid integrity RT-qPCR for the selective detection of intact SARS-CoV-2 in wastewater.The Science of the total environment · 2021Article
- Exploiting Complex Fluorophore Interactions to Monitor Virus Capsid Disassembly.Molecules (Basel, Switzerland) · 2021Article
- A new long-term sampling approach to viruses on surfaces.Scientific reports · 2021Article
- Detection and evaluation of rotavirus surveillance methods as viral indicator in the aquatic environments.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- SARS-CoV-2 in water services: Presence and impacts.Environmental pollution (Barking, Essex : 1987) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, a wide range of analytical methods is available for virus detection in environmental water samples. Molecular methods such as polymerase chain reaction (PCR) and quantitative real time PCR (qPCR) have the highest sensitivity and specificity to investigate virus contamination in water, so they are the most commonly used in environmental virology. Despite great sensitivity of PCR, the main limitation is the lack of the correlation between the detected viral genome and viral infectivity, which limits conclusions regarding the significance for public health. To provide information about the infectivity of the detected viruses, cultivation on animal cell culture is the gold standard. However, cell culture infectivity assays are laborious, time consuming and costly. Also, not all viruses are able to produce cytopathic effect and viruses such as human noroviruses have no available cell line for propagation. In this brief review, we present a summary and critical evaluation of different approaches that have been recently proposed to overcome limitations of the traditional cell culture assay and PCR assay such as integrated cell culture-PCR, detection of genome integrity, detection of capsid integrity, and measurement of oxidative damages on viral capsid protein. Techniques for rapid detection of infectious viruses such as fluorescence microscopy and automated flow cytometry have also been suggested to assess virus infectivity in water samples.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.