ArticleBrain pathology (Zurich, Switzerland)2012
Use of murine bioassay to resolve ovine transmissible spongiform encephalopathy cases showing a bovine spongiform encephalopathy molecular profile.
Article in Brain pathology (Zurich, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Oral Transmission of Classical Bovine Spongiform Encephalopathy in ARR/ARR Sheep.Emerging infectious diseases · 2025Article
- Host prion protein expression levels impact prion tropism for the spleen.PLoS pathogens · 2020Article
- Characterization of goat prions demonstrates geographical variation of scrapie strains in Europe and reveals the composite nature of prion strains.Scientific reports · 2020Article
- Re-transmissibility of mouse-adapted ME7 scrapie strain to ovine PrP transgenic mice.Journal of veterinary science · 2019Article
- Pathology of Animal Transmissible Spongiform Encephalopathies (TSEs).Food safety (Tokyo, Japan) · 2017Review
- Selective propagation of mouse-passaged scrapie prions with long incubation period from a mixed prion population using GT1-7 cells.PloS one · 2017Article
- Primary transmission of chronic wasting disease versus scrapie prions from small ruminants to transgenic mice expressing ovine or cervid prion protein.The Journal of general virology · 2016Article
- Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrP(Sc) over time.Veterinary research · 2015Article
- Ability of wild type mouse bioassay to detect bovine spongiform encephalopathy (BSE) in the presence of excess scrapie.Acta neuropathologica communications · 2015Article
- Disease characteristics of bovine spongiform encephalopathy following inoculation into mice via three different routes.International journal of experimental pathology · 2013Article
- Evidence in sheep for pre-natal transmission of scrapie to lambs from infected mothers.PloS one · 2013Article
- Article
- The interpretation of disease phenotypes to identify TSE strains in mice: characterisation of BSE using PrPSc distribution patterns in the brain.Veterinary research · 2012Article
- The interpretation of disease phenotypes to identify TSE strains following murine bioassay: characterisation of classical scrapie.Veterinary research · 2012Article
- Transmissibility of caprine scrapie in ovine transgenic mice.BMC veterinary research · 2012Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two cases of unusual transmissible spongiform encephalopathy (TSE) were diagnosed on the same farm in ARQ/ARQ PrP sheep showing attributes of both bovine spongiform encephalopathy (BSE) and scrapie. These cases, UK-1 and UK-2, were investigated further by transmissions to wild-type and ovine transgenic mice. Lesion profiles (LP) on primary isolation and subpassage, incubation period (IP) of disease, PrP(Sc) immunohistochemical (IHC) deposition pattern and Western blot profiles were used to characterize the prions causing disease in these sheep. Results showed that both cases were compatible with scrapie. The presence of BSE was contraindicated by the following: LP on primary isolation in RIII and/or MR (modified RIII) mice; IP and LP after serial passage in wild-type mice; PrP(Sc) deposition pattern in wild-type mice; and IP and Western blot data in transgenic mice. Furthermore, immunohistochemistry (IHC) revealed that each case generated two distinct PrP(Sc) deposition patterns in both wild-type and transgenic mice, suggesting that two scrapie strains coexisted in the ovine hosts. Critically, these data confirmed the original differential IHC categorization that these UK-1 and UK-2 cases were not compatible with BSE.
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Registered trials
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