ArticleYeast (Chichester, England)2011
Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3 mutant.
Article in Yeast (Chichester, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Functional characterization of human recessive DIS3 variants in premature ovarian insufficiency†.Biology of reproduction · 2025Article
- Article
- Landscape of functional interactions of human processive ribonucleases revealed by high-throughput siRNA screenings.iScience · 2021Article
- The RNA Exosome and Human Disease.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Exonuclease domain mutants of yeast DIS3 display genome instability.Nucleus (Austin, Tex.) · 2019Article
- Article
- Loss of the RNA helicase SKIV2L2 impairs mitotic progression and replication-dependent histone mRNA turnover in murine cell lines.RNA (New York, N.Y.) · 2017Article
- Functional characterization of a chr13q22.1 pancreatic cancer risk locus reveals long-range interaction and allele-specific effects on DIS3 expression.Human molecular genetics · 2016Article
- The 3' to 5' Exoribonuclease DIS3: From Structure and Mechanisms to Biological Functions and Role in Human Disease.Biomolecules · 2015Review
- The RNA exosome affects iron response and sensitivity to oxidative stress.RNA (New York, N.Y.) · 2014Article
- Mechanisms of genome instability induced by RNA-processing defects.Trends in genetics : TIG · 2014Review
- An evolutionarily conserved RNase-based mechanism for repression of transcriptional positive autoregulation.Molecular microbiology · 2014Article
- The ribonuclease Dis3 is an essential regulator of the developmental transcriptome.BMC genomics · 2012Article
- Dis3- and exosome subunit-responsive 3' mRNA instability elements.Biochemical and biophysical research communications · 2012Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Subunits of the RNA processing exosome assemble into structurally distinct protein complexes that function in disparate cellular compartments and RNA metabolic pathways. Here, in a genetic, cell biological and transcriptomic analysis, we examined the role of Dis3, an essential polypeptide with endo- and 3'→5' exo-ribonuclease activity, in cell cycle progression. We present several lines of evidence that perturbation of DIS3 affects microtubule (MT) localization and structure in Saccharomyces cerevisiae. Cells with a DIS3 mutant: (a) accumulate anaphase and pre-anaphase mitotic spindles; (b) exhibit spindles that are misorientated and displaced from the bud neck; (c) harbour elongated spindle-associated astral MTs; (d) have an increased G1 astral MT length and number; and (e) are hypersensitive to MT poisons. Mutations in the core exosome genes RRP4 and MTR3 and the exosome cofactor gene MTR4, but not other exosome subunit gene mutants, also elicit MT phenotypes. RNA deep sequencing analysis (RNA-seq) shows broad changes in the levels of cell cycle- and MT-related transcripts in mutant strains. Collectively, the data presented in this study suggest an evolutionarily conserved role for Dis3 in linking RNA metabolism, MTs and cell cycle progression.
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Registered trials
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