ArticleExtremophiles : life under extreme conditions2011
Cloning and characterization of the TneDI restriction: modification system of Thermotoga neapolitana.
Article in Extremophiles : life under extreme conditions, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 10 citations in OpenAlex.
- Thermosipho spp. Immune System Differences Affect Variation in Genome Size and Geographical Distributions.Genome biology and evolution · 2018Article
- Complete genome sequence ofStandards in genomic sciences · 2017Article
- Expression of Heterologous Cellulases in Thermotoga sp. Strain RQ2.BioMed research international · 2015Article
- Natural transformation of Thermotoga sp. strain RQ7.BMC biotechnology · 2014Article
- Construction and transformation of a Thermotoga-E. coli shuttle vector.BMC biotechnology · 2012Article
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4 authors at 1 institution in 1 country.
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Abstract
A putative Type II restriction-modification system of Thermotoga neapolitana, TneDI, was cloned into Escherichia coli XL1-Blue MRF' and characterized. Gene CTN_0339 specifies the endonuclease R.TneDI, while CTN_0340 encodes the cognate DNA methyltransferase M.TneDI. Both enzymes were purified simply by heating the cell lysates of E. coli followed by centrifugation. The enzymes were active over a broad range of temperatures, from 42°C to at least 77°C, with the highest activities observed at 77°C. R.TneDI cleaved at the center of the recognition sequence (CG↓CG) and generated blunt-end cuts. Overexpression of R.TneDI in BL21(DE3) was confirmed by both SDS-PAGE and Western blotting.
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