ArticleJournal of immunology (Baltimore, Md. : 1950)2011
Myeloid differentiation factor-2 interacts with Lyn kinase and is tyrosine phosphorylated following lipopolysaccharide-induced activation of the TLR4 signaling pathway.
Article in Journal of immunology (Baltimore, Md. : 1950), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Interferon regulatory factor 5: a potential target for therapeutic intervention in inflammatory diseases.Frontiers in immunology · 2025Review
- Neuroprotective efficiency of celecoxib vesicular bilosomes for the management of lipopolysaccharide-induced Alzheimer in mice employing 2Inflammopharmacology · 2024Article
- An insight into the neuroprotective and anti-neuroinflammatory effects and mechanisms ofFrontiers in pharmacology · 2022Review
- Effect of Food Endotoxin on Infant Health.Toxins · 2021Review
- Lipopolysaccharide-Induced Neuroinflammation as a Bridge to Understand Neurodegeneration.International journal of molecular sciences · 2019Review
- Advances and challenges in targeting IRF5, a key regulator of inflammation.The FEBS journal · 2019Review
- Alternatively spliced myeloid differentiation protein-2 inhibits TLR4-mediated lung inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- GADD34 inhibits activation-induced apoptosis of macrophages through enhancement of autophagy.Scientific reports · 2015Article
- Co-operation of TLR4 and raft proteins in LPS-induced pro-inflammatory signaling.Cellular and molecular life sciences : CMLS · 2015Review
- TGFβ receptor 1: an immune susceptibility gene in HPV-associated cancer.Cancer research · 2014Article
- Tyrosine phosphorylation in Toll-like receptor signaling.Cytokine & growth factor reviews · 2014Review
- Radioiodination of an endotoxin·MD-2 complex generates a novel sensitive, high-affinity ligand for TLR4.Innate immunity · 2013Article
- Identification of macrophage genes responsive to extracellular acidification.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2013Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Stimulation with LPS induces tyrosine phosphorylation of numerous proteins involved in the TLR signaling pathway. In this study, we demonstrated that myeloid differentiation factor-2 (MD-2) is also tyrosine phosphorylated following LPS stimulation. LPS-induced tyrosine phosphorylation of MD-2 is specific; it is blocked by the tyrosine kinase inhibitor, herbimycin A, as well as by an inhibitor of endocytosis, cytochalasin D, suggesting that MD-2 phosphorylation occurs during trafficking of MD-2 and not on the cell surface. Furthermore, we identified two possible phospho-accepting tyrosine residues at positions 22 and 131. Mutant proteins in which these tyrosines were changed to phenylalanine had reduced phosphorylation and significantly diminished ability to activate NF-κB in response to LPS. In addition, MD-2 coprecipitated and colocalized with Lyn kinase, most likely in the endoplasmic reticulum. A Lyn-binding peptide inhibitor abolished MD-2 tyrosine phosphorylation, suggesting that Lyn is a likely candidate to be the kinase required for MD-2 tyrosine phosphorylation. Our study demonstrated that tyrosine phosphorylation of MD-2 is important for signaling following exposure to LPS and underscores the importance of this event in mediating an efficient and prompt immune response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.