ArticleClinical and vaccine immunology : CVI2011
Development and evaluation of an enzyme-linked immunosorbent assay for use in the detection of bovine tuberculosis in cattle.
Article in Clinical and vaccine immunology : CVI, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Accuracy of Tests for Diagnosis of Animal Tuberculosis: Moving Away from the Golden Calf (and towards Bayesian Models).Transboundary and emerging diseases · 2023Pooled it
- Recombinant proteins expressed in Mycolicibacterium smegmatis enhance antibody-based detection of bovine tuberculosis.Veterinary research · 2026Article
- Proteome microarray-guided identification of mycobacterial antigens and ELISA-based peptide mapping for improved serological detection ofJournal of clinical microbiology · 2026Article
- The Impact of Bacillus Calmette-Guérin Vaccination andVaccines · 2025Article
- Re-Evaluation of ELISA for the Detection of Bovine Tuberculosis and a New Proposal for Its Use in Eradication Efforts on Outbreak Farms.Pathogens (Basel, Switzerland) · 2025Article
- Evaluation of serological assays forFrontiers in microbiology · 2025Article
- Field evaluation of the P22 ELISA for diagnosis of caprine tuberculosis in an endemic area.Frontiers in veterinary science · 2025Article
- B Cell and Antibody Responses in Bovine Tuberculosis.Antibodies (Basel, Switzerland) · 2024Review
- Susceptibility, Immunity, and Persistent Infection Drive Endemic Cycles of Coxiellosis on Dairy Farms.Animals : an open access journal from MDPI · 2024Article
- Temporal dynamics of the early immune response following Mycobacterium bovis infection of cattle.Scientific reports · 2024Article
- Diagnostic accuracy of the Enferplex Bovine TB antibody test using individual milk samples from cattle.PloS one · 2024Article
- Limited usefulness of the ISFrontiers in veterinary science · 2024Article
- Article
- Is Serological Monitoring a Fit-for-purpose Tool to Assess the Epidemiological Situation of Tuberculosis in the Sylvatic Species of European Bison (Journal of veterinary research · 2022Article
- A combined application of molecular docking technology and indirect ELISA for the serodiagnosis of bovine tuberculosis.Journal of veterinary science · 2022Article
- Study on supplemental test to improve the detection of bovine tuberculosis in individual animals and herds.BMC veterinary research · 2021Article
- Comparing recombinant MPB70/SahH and native 20-kDa protein for detecting bovine tuberculosis using ELISA.The Journal of veterinary medical science · 2020Article
- Review
- Determination of the sensitivity and specificity of bovine tuberculosis screening tests in dairy herds in Thailand using a Bayesian approach.BMC veterinary research · 2019Article
- Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations.BMC veterinary research · 2019Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a consequence of continued spillover of Mycobacterium bovis into cattle from wildlife reservoirs and increased globalization of cattle trade with associated transmission risks, new approaches such as vaccination and novel testing algorithms are seriously being considered by regulatory agencies for the control of bovine tuberculosis. Serologic tests offer opportunities for identification of M. bovis-infected animals not afforded by current diagnostic techniques. The present study describes assay development and field assessment of a new commercial enzyme-linked immunosorbent assay (ELISA) that detects antibody to M. bovis antigens MPB83 and MPB70 in infected cattle. Pertinent findings include the following: specific antibody responses were detected at ∼90 to 100 days after experimental M. bovis challenge, minimal cross-reactive responses were elicited by infection/sensitization with nontuberculous Mycobacterium spp., and the apparent sensitivity and specificity of the ELISA with naturally infected cattle were 63% and 98%, respectively, with sensitivity improving as disease severity increased. The ELISA also detected infected animals missed by the routine tuberculin skin test, and antibody was detectable in bulk tank milk samples from M. bovis-infected dairy herds. A high-throughput ELISA could be adapted as a movement, border, or slaughter surveillance test, as well as a supplemental test to tuberculin skin testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.