ArticleJournal of clinical microbiology2011
Diagnosis of Listeria monocytogenes meningoencephalitis by real-time PCR for the hly gene.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 100 citations in OpenAlex.
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- Metagenomic detection of central nervous system infections missedby conventional testing.JCI insight · 2025Article
- Exploration of the biodiversity and mining novel target genes ofFrontiers in microbiology · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Clinical and microbiological characteristics and follow-up of invasive Listeria monocytogenes infection among hospitalized patients: real-world experience of 16 years from Hungary.BMC microbiology · 2024Observational
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- Imaging modalities in neurolisteriosis: a literature review.Polish journal of radiology · 2024Review
- Brain abscesses caused byLe infezioni in medicina · 2023Article
- Bacterial meningitis in Africa.Frontiers in neurology · 2023Review
- Cerebrospinal fluid analysis: current diagnostic methods in central nervous system infectious diseases.Arquivos de neuro-psiquiatria · 2022Article
- Listeria monocytogenes faecal carriage is common and depends on the gut microbiota.Nature communications · 2021Article
- Immunocompromised Child on Infliximab: A Case Report of Listeria monocytogenes Meningitis.Clinical practice and cases in emergency medicine · 2020Article
- Presence ofMicroorganisms · 2020Article
- Detection of Listeria monocytogenes in a patient with meningoencephalitis using next-generation sequencing: a case report.BMC infectious diseases · 2020Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is a bacterial pathogen that can invade the central nervous system (CNS), causing meningoencephalitis and brain abscesses. The diagnosis of CNS listeriosis, based on the isolation of the bacteria in the cerebrospinal fluid (CSF), can be difficult because of previous antibiotic treatment and a low number of bacteria in the CSF. To improve the sensitivity of microbiological diagnosis, we have developed a real-time PCR assay for detecting and quantifying L. monocytogenes DNA in the CSF. The designed primers specifically amplify the L. monocytogenes hly gene, which encodes listeriolysin O, a pore-forming cytolysin. The PCR assay for the hly gene (PCR-hly) provides reproducible quantitative results over a wide dynamic range of concentrations and was highly sensitive while detecting a single gene copy/ml. By assaying a large panel of bacterial species, including species secreting pore-forming cytolysin, we determined the specificity of the PCR-hly, which exclusively detects the L. monocytogenes DNA. We then analyzed 214 CSF samples from patients suspected of having CNS listeriosis. PCR-hly was positive in all cases in which L. monocytogenes was isolated by culture. Positive PCR-hly of the CSF was also obtained for five additional, clinically confirmed cases of CNS listeriosis for which bacterial cultures were negative presumably due to previous treatment with antibiotics. As a complement to classical bacteriological CSF culture, our designed real-time PCR-hly assay proved to be valuable by enhancing the rapidity and the accuracy of the diagnosis of CNS infection by L. monocytogenes. In addition, the quantitative results provided may, in some instances, be useful for the follow-up of patients under treatment.
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