ArticleNucleic acids research2012
Single-nucleotide base excision repair DNA polymerase activity in C. elegans in the absence of DNA polymerase β.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Two distinct Okazaki fragment failure modes dominate mutagenesis in the absence of flap endonuclease 1.Nature communications · 2026Article
- A new Caenorhabditis elegans apurinic/apyrimidinic (AP) endonuclease engaged in rescue from replication stress-induced arrest.Genetics and molecular biology · 2025Article
- Genome Protection by DNA Polymerase θ.Annual review of genetics · 2022Review
- Review
- The base excision repair process: comparison between higher and lower eukaryotes.Cellular and molecular life sciences : CMLS · 2021Review
- The role of poly(ADP-ribose) polymerases in manganese exposed Caenorhabditis elegans.Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2020Article
- The Base Excision Repair Pathway in the NematodeFrontiers in cell and developmental biology · 2020Review
- Motor neuron disease-associated loss of nuclear TDP-43 is linked to DNA double-strand break repair defects.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- UNG-1 and APN-1 are the major enzymes to efficiently repair 5-hydroxymethyluracil DNA lesions in C. elegans.Scientific reports · 2018Article
- DNA polymerase θ (POLQ), double-strand break repair, and cancer.DNA repair · 2016Review
- Nucleosomes determine their own patch size in base excision repair.Scientific reports · 2016Article
- A putative Leishmania DNA polymerase theta protects the parasite against oxidative damage.Nucleic acids research · 2016Article
- Elemental bioimaging of Cisplatin in Caenorhabditis elegans by LA-ICP-MS.Metallomics : integrated biometal science · 2015Article
- Human DNA polymerase θ grasps the primer terminus to mediate DNA repair.Nature structural & molecular biology · 2015Article
- A DNA repair variant in POLQ (c.-1060A > G) is associated to hereditary breast cancer patients: a case-control study.BMC cancer · 2014Article
- Article
- Article
- DNA polymerase POLQ and cellular defense against DNA damage.DNA repair · 2013Review
- Pol β associated complex and base excision repair factors in mouse fibroblasts.Nucleic acids research · 2012Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The base excision DNA repair (BER) pathway known to occur in Caenorhabditis elegans has not been well characterized. Even less is known about the DNA polymerase (pol) requirement for the gap-filling step during BER. We now report on characterization of in vitro uracil-DNA initiated BER in C. elegans. The results revealed single-nucleotide (SN) gap-filling DNA polymerase activity and complete BER. The gap-filling polymerase activity was not due to a DNA polymerase β (pol β) homolog, or to another X-family polymerase, since computer-based sequence analyses of the C. elegans genome failed to show a match for a pol β-like gene or other X-family polymerases. Activity gel analysis confirmed the absence of pol β in the C. elegans extract. BER gap-filling polymerase activity was partially inhibited by both dideoxynucleotide and aphidicolin. The results are consistent with a combination of both replicative polymerase(s) and lesion bypass/BER polymerase pol θ contributing to the BER gap-filling synthesis. Involvement of pol θ was confirmed in experiments with extract from pol θ null animals. The presence of the SN BER in C. elegans is supported by these results, despite the absence of a pol β-like enzyme or other X-family polymerase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.