ArticleParasites & vectors2011
Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool.
Article in Parasites & vectors, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
- Conventional and Advanced Methods Used for the Diagnosis of Fascioliosis, a Food-Borne Zoonotic Disease.Journal of parasitology research · 2025Review
- Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep.PloS one · 2022Article
- Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis.Acta parasitologica · 2021Review
- Single Domain Antibodies as New Biomarker Detectors.Diagnostics (Basel, Switzerland) · 2017Review
- Immunodiagnosis of Human Fascioliasis: An Update of Concepts and Performances of the Serological Assays.Journal of clinical and diagnostic research : JCDR · 2017Review
- Immunolocalization and immunodetection of the excretory/secretory (ES) antigens of Fasciola gigantica.PloS one · 2017Article
- Serodiagnosis of fasciolosis by fast protein liquid chromatography-fractionated excretory/secretory antigens.Parasitology research · 2016Article
- Article
- Evaluation of antibody response to various developmental stage specific somatic antigens of Paramphistomum epiclitum in goats.BioMed research international · 2014Article
- Immunodetection of Fasciola gigantica circulating antigen in sera of infected individuals for laboratory diagnosis of human fascioliasis.Clinical and vaccine immunology : CVI · 2013Article
- The global burden of fasciolosis in domestic animals with an outlook on the contribution of new approaches for diagnosis and control.Parasitology research · 2013Review
- Molecular, biochemical, and morphometric characterization of Fasciola species potentially causing zoonotic disease in Egypt.Parasitology research · 2012Article
- Development and evaluation of a single-step duplex PCR for simultaneous detection of Fasciola hepatica and Fasciola gigantica (family Fasciolidae, class Trematoda, phylum Platyhelminthes).Journal of clinical microbiology · 2012Article
- Detection of microorganisms in granulomas that have been formalin-fixed: review of the literature regarding use of molecular methods.Scientifica · 2012Review
- Performance of a 27 kDa Fasciola hepatica Antigen in the Diagnosis of Human Fascioliasis.Journal of laboratory physiciansArticle
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9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis research was carried out to develop a reliable monoclonal antibody (MoAb)-based sandwich enzyme linked immunosorbent assay (ELISA) for the diagnosis of active Fasciola gigantica infection in both serum and stool for comparative purposes.
methodsFrom a panel of MoAbs raised against F. gigantica excretory/secretory antigens (ES Ags), a pair (12B/11D/3F and 10A/9D/10G) was chosen due to its high reactivity and strict specificity to F. gigantica antigen by indirect ELISA.
resultsThe two MoAbs were of the IgG₁ and IgG(₂a) subclasses, respectively. Using SDS-PAGE and EITB, the selected MoAbs recognized 83, 64, 45 and 26 kDa bands of ES Ags. The lower detection limit of ELISA assay was 3 ng/ml. In stool, the sensitivity, specificity and diagnostic efficacy of ELISA was 96%, 98.2 and 97.1%; while in serum they were 94%, 94.6% and 94.3%, respectively. Moreover, a positive correlation was found between ova count in stool of F. gigantica infected patients and the OD readings of ELISA in both stool and serum samples (r = 0.730, p < 0.01 and r = 0.608; p < 0.01, respectively).
conclusionsThese data showed that the use of MoAb-based sandwich ELISA for the detection of F. gigantica coproantigens in stool specimens was superior to serum samples; it provides a highly efficient, non-invasive technique for the diagnosis of active F. gigantica infection.
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