ArticleEcotoxicology (London, England)2012
Construction and characterization of a normalized cDNA library from the river snail Bellamya aeruginosa after exposure to copper.
Article in Ecotoxicology (London, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Population Genomic Evidence for the Diversification ofBiology · 2022Article
- Large-Scale Investigation of Soybean Gene Functions by Overexpressing a Full-Length Soybean cDNA Library inFrontiers in plant science · 2018Article
- Estrogen alters the profile of the transcriptome in river snail Bellamya aeruginosa.Ecotoxicology (London, England) · 2015Article
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Authors and funding
10 authors.
Funding
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Abstract
The construction of a normalized cDNA library is a popular tool for identifying novel biomarkers for monitoring environmental pollution. In the present study, a normalized cDNA library was constructed from the river snail Bellamya aeruginosa after exposure to Cu(2+) by using the SMART technique. The titer of the cDNA library was 1.78 × 10(6) pfu/ml, with a recombinant efficiency of 95.8%. In addition, from 6,000 randomly selected and sequenced clones, 5,473 high-quality ESTs were identified. After processing the sequences, 3,961 unigenes representing 897 contigs and 3,064 singlets were obtained with 27.6% redundancy. Analysis of expressed sequenced tags using COG and GO annotation and KEGG pathway data showed that a large group of genes related to growth and development, signal transduction, and defense mechanisms were present in the cDNA library. Based on our findings, this normalized cDNA library will provide a valuable resource for further research on functional genes and ecotoxicology in B. aeruginosa.
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