ArticleCytotechnology2011
Bone marrow mesenchymal stem cells differentiate into urothelial cells and the implications for reconstructing urinary bladder mucosa.
Article in Cytotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- A novel tubular construct using silk fibroin-chitosan scaffold seeded with bone marrow mesenchymal stem cells for urinary diversion in a preclinical rabbit model.Frontiers in surgery · 2026Article
- Mechanism of the enterobacterial metabolite sodium butyrate mediating ferroptosis to affect osteogenic ability of BMSCs in mice with estrogen deficiency-caused osteoporosis via the PTEN/PI3K/AKT pathway.Apoptosis : an international journal on programmed cell death · 2025Article
- Strategies of Bladder Reconstruction after Partial or Radical Cystectomy for Bladder Cancer.Molecular biotechnology · 2025Review
- A Novel Hybrid Membrane for Urinary Conduit Substitutes Based on Small Intestinal Submucosa Coupled with Two Synthetic Polymers.Journal of functional biomaterials · 2022Article
- The urothelium: a multi-faceted barrier against a harsh environment.Mucosal immunology · 2022Review
- Hydrogel composite scaffolds achieve recruitment and chondrogenesis in cartilage tissue engineering applications.Journal of nanobiotechnology · 2022Article
- Non-Muscular Invasive Bladder Cancer: Re-envisioning Therapeutic Journey from Traditional to Regenerative Interventions.Aging and disease · 2021Review
- Urethroplasty performed with an autologous urothelium-vegetated collagen fleece to treat urethral stricture in the minipig model.World journal of urology · 2020Article
- Application of Adult and Pluripotent Stem Cells in Interstitial Cystitis/Bladder Pain Syndrome Therapy: Methods and Perspectives.Journal of clinical medicine · 2020Review
- Review
- Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder.Scientific reports · 2019Article
- Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction.Stem cell research & therapy · 2018Article
- Mesenchymal Stem Cells: Miraculous Healers or Dormant Killers?Stem cell reviews and reports · 2018Article
- Differential transcription factor expression by human epithelial cells of buccal and urothelial derivation.Experimental cell research · 2018Article
- Stem cell in urology-are we at the cusp of a new era?Translational andrology and urology · 2018Review
- Review
- Differentiate into urothelium and smooth muscle cells from adipose tissue-derived stem cells for ureter reconstruction in a rabbit model.American journal of translational research · 2016Article
- Dynamic reciprocity in cell-scaffold interactions.Advanced drug delivery reviews · 2015Review
- Production of urothelium from pluripotent stem cells for regenerative applications.Current urology reports · 2015Review
- Transdifferentiation of autologous bone marrow cells on a collagen-poly(ε-caprolactone) scaffold for tissue engineering in complete lack of native urothelium.Journal of the Royal Society, Interface · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine the ability of cultured bone marrow-derived mesenchymal stem cells (BMSCs) to differentiate into functional urothelium. BMSCs were isolated from the long bones of aborted fetal limbs by Percoll density gradient centrifugation and characterized by flow cytometry. Human fetal urinary bladders were cut into small pieces and cultured for 3-5 days until the growth of urothelial cells was established. BMSCs were then cocultured with neonatal urothelial cells and subsequently evaluated for antigen expression and ultramicrostructure, by immunocytochemistry and electron microscopy, respectively. A subset of BMSCs expressed the differentiation marker CD71. The BMSC markers CD34, CD45, and HLA-DR were barely detectable, confirming that these cells were not derived from hematopoietic stem cells or differentiated cells. In contrast, the stem cell markers CD29, CD44, CD105, and CD90 were highly expressed. BMSCs possessed the ability to differentiate into a variety of cellular subtypes, including osteocytes, adipocytes, and chondrocytes. The shapes of BMSCs changed, and the size of the cells increased, following in vitro coculture with urothelial cells. After 2 weeks of coculture, immunostaining of the newly differentiated BMSCs positively displayed the urothelial-specific keratin marker. Electron microscopy revealed that the cocultured BMSCs had microstructural features characteristic of epithelial cells. Pluripotent BMSCs can transdifferentiate into urothelial cells in response to an environment conditioned by neonatal urothelial cells, providing a means for the time-, labor- and cost-effective reconstruction of urinary bladder mucosa.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.