ArticlePloS one2011
Seamless gene tagging by endonuclease-driven homologous recombination.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
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- High-throughput functional characterization of protein phosphorylation sites in yeast.Nature biotechnology · 2022Article
- Spatial control of avidity regulates initiation and progression of selective autophagy.Nature communications · 2021Article
- Timer-based proteomic profiling of the ubiquitin-proteasome system reveals a substrate receptor of the GID ubiquitin ligase.Molecular cell · 2021Article
- Exploring whole-genome duplicate gene retention with complex genetic interaction analysis.Science (New York, N.Y.) · 2020Article
- The protein architecture of the endocytic coat analyzed by FRET microscopy.Molecular systems biology · 2020Article
- The functional landscape of the human phosphoproteome.Nature biotechnology · 2020Article
- Tricalbins Contribute to Cellular Lipid Flux and Form Curved ER-PM Contacts that Are Bridged by Rod-Shaped Structures.Developmental cell · 2019Article
- FRET Microscopy in Yeast.Biosensors · 2019Review
- Nuclear pores as versatile reference standards for quantitative superresolution microscopy.Nature methods · 2019Article
- Cooperation of mitochondrial and ER factors in quality control of tail-anchored proteins.eLife · 2019Article
- Conserved phosphorylation hotspots in eukaryotic protein domain families.Nature communications · 2019Article
- Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.Current genetics · 2018Article
- Reconstitution reveals Ykt6 as the autophagosomal SNARE in autophagosome-vacuole fusion.The Journal of cell biology · 2018Article
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Authors and funding
5 authors.
Funding
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Abstract
Gene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here we describe a seamless gene tagging approach that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-strand break repair by homologous recombination. We implement seamless tagging in Saccharomyces cerevisiae and demonstrate its application for protein tagging while preserving simultaneously upstream and downstream gene regulatory elements. Seamless tagging is compatible with high-throughput strain construction using synthetic genetic arrays (SGA), enables functional analysis of transcription antisense to open reading frames and should facilitate systematic and minimally-invasive analysis of gene functions.
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