ArticlePlastic and reconstructive surgery2012
Enhancement of human adipose-derived stromal cell angiogenesis through knockdown of a BMP-2 inhibitor.
Article in Plastic and reconstructive surgery, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Adipose Tissue-derived Stem cells in Plastic and Reconstructive Surgery: A Bibliometric Study.Aesthetic plastic surgery · 2021Pooled it
- Magnetic resonance imaging of stem cell-macrophage interactions with ferumoxytol and ferumoxytol-derived nanoparticles.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- Comparison of osteogenic differentiation induced byInternational journal of nanomedicine · 2019Article
- Review
- Human Mesenchymal Stem Cells Genetically Engineered to Overexpress Brain-derived Neurotrophic Factor Improve Outcomes in Huntington's Disease Mouse Models.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- Translational treatment paradigm for managing non-unions secondary to radiation injury utilizing adipose derived stem cells and angiogenic therapy.Head & neck · 2016Article
- Gene profile of soluble growth factors involved in angiogenesis, in an adipose-derived stromal cell/endothelial cell co-culture, 3D gel model.Cell proliferation · 2015Article
- Engendering allograft ignorance in a mouse model of allogeneic skin transplantation to the distal hind limb.Annals of surgery · 2015Article
- Cell recruitment by amnion chorion grafts promotes neovascularization.The Journal of surgical research · 2015Article
- In vitro evaluation of rhBMP-2-induced expression of VEGF in human adipose-derived stromal cells.International journal of clinical and experimental medicine · 2015Article
- Burn injury enhances bone formation in heterotopic ossification model.Annals of surgery · 2014Article
- The roles of bone morphogenetic proteins and their signaling in the osteogenesis of adipose-derived stem cells.Tissue engineering. Part B, Reviews · 2014Review
- Identical effects of VEGF and serum-deprivation on phenotype and function of adipose-derived stromal cells from healthy donors and patients with ischemic heart disease.Journal of translational medicine · 2013Article
- Notochordal cell-derived therapeutic strategies for discogenic back pain.Global spine journal · 2013Review
- Article
- In vivo directed differentiation of pluripotent stem cells for skeletal regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundPrevious studies have demonstrated the role of noggin, a bone morphogenetic protein-2 inhibitor, in vascular development and angiogenesis. The authors hypothesized that noggin suppression in human adipose-derived stromal cells would enhance vascular endothelial growth factor secretion and angiogenesis in vitro and in vivo to a greater extent than bone morphogenetic protein-2 alone.
methodsHuman adipose-derived stromal cells were isolated from human lipoaspirate (n = 6) noggin was knocked down using lentiviral techniques. Knockdown was confirmed and angiogenesis was assessed by tubule formation and quantitative real-time polymerase chain reaction. Cells were seeded onto scaffolds and implanted into a 4-mm critical size calvarial defect. In vivo angiogenic signaling was assessed by immunofluorescence and immunohistochemistry.
resultsHuman adipose-derived stromal cells with noggin suppression secreted significantly higher amounts of angiogenic proteins, expressed higher levels of angiogenic genes, and formed more tubules in vitro. In vivo, calvarial defects seeded with noggin shRNA human adipose-derived stromal cells exhibited a significantly higher number of vessels in the defect site than controls by immunohistochemistry (p < 0.05). In addition, bone morphogenetic protein-2-releasing scaffolds significantly enhanced vascular signaling in the defect site.
conclusionsHuman adipose-derived stromal cells demonstrate significant increases in angiogenesis in vitro and in vivo with both noggin suppression and BMP-2 supplementation. By creating a cell with noggin suppressed and by using a scaffold with increased bone morphogenetic protein-2 signaling, a more angiogenic niche can be created.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.