ArticleProceedings of the National Academy of Sciences of the United States of America2011
Design and application of a class of sensors to monitor Ca2+ dynamics in high Ca2+ concentration cellular compartments.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
61 citing papers in PubMed.
- The Multifaceted Role of Calcium Signaling in Regulated Necrosis.Biomolecules · 2025Review
- Highly dynamic and sensitive NEMOer calcium indicators for imaging ER calcium signals in excitable cells.Nature communications · 2025Article
- Review
- Self-Assembly of Microstructured Protein Coatings with Programmable Functionality for Fluorescent Biosensors.ACS applied materials & interfaces · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives.Plant physiology · 2023Review
- Alternative Strategy for Spectral Tuning of Flavin-Binding Fluorescent Proteins.The journal of physical chemistry. B · 2023Article
- The evolution of organellar calcium mapping technologies.Cell calcium · 2022Review
- Shaped by leaky ER: Homeostatic CaFrontiers in physiology · 2022Review
- Extracellular calcium alters calcium-sensing receptor network integrating intracellular calcium-signaling and related key pathway.Scientific reports · 2021Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- A new set of equations for the simplified calibration of fluorescence CaJournal of muscle research and cell motility · 2021Article
- The emerging role of the sympathetic nervous system in skeletal muscle motor innervation and sarcopenia.Ageing research reviews · 2021Review
- Article
- Incorporation of sensing modalities into de novo designed fluorescence-activating proteins.Nature communications · 2021Article
- Starch-Based Carbon Dots for Nitrite and Sulfite Detection.Frontiers in chemistry · 2021Article
- Design of Calcium-Binding Proteins to Sense Calcium.Molecules (Basel, Switzerland) · 2020Review
- High-throughput-compatible assays using a genetically-encoded calcium indicator.Scientific reports · 2019Article
- Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification.International journal of molecular sciences · 2019Review
- A mathematical model of calcium dynamics: Obesity and mitochondria-associated ER membranes.PLoS computational biology · 2019Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Quantitative analysis of Ca(2+) fluctuations in the endoplasmic/sarcoplasmic reticulum (ER/SR) is essential to defining the mechanisms of Ca(2+)-dependent signaling under physiological and pathological conditions. Here, we developed a unique class of genetically encoded indicators by designing a Ca(2+) binding site in the EGFP. One of them, calcium sensor for detecting high concentration in the ER, exhibits unprecedented Ca(2+) release kinetics with an off-rate estimated at around 700 s(-1) and appropriate Ca(2+) binding affinity, likely attributable to local Ca(2+)-induced conformational changes around the designed Ca(2+) binding site and reduced chemical exchange between two chromophore states. Calcium sensor for detecting high concentration in the ER reported considerable differences in ER Ca(2+) dynamics and concentration among human epithelial carcinoma cells (HeLa), human embryonic kidney 293 cells (HEK-293), and mouse myoblast cells (C2C12), enabling us to monitor SR luminal Ca(2+) in flexor digitorum brevis muscle fibers to determine the mechanism of diminished SR Ca(2+) release in aging mice. This sensor will be invaluable in examining pathogenesis characterized by alterations in Ca(2+) homeostasis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.