ReviewJournal of applied genetics2011
TILLING: a shortcut in functional genomics.
Review in Journal of applied genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
83 citing papers in PubMed, 231 citations in OpenAlex.
- Wheat GT-1-like Transcription Factor Boosts Cutin Biosynthesis.Biomolecules · 2026Article
- Wheat Biofortification for Enhancing Iron, Zinc, and Protein: The Role of Mutation Breeding.Plants (Basel, Switzerland) · 2026Review
- Wheat MYB46-like Transcription Factor Stimulates Cuticular Wax Biosynthesis.Biomolecules · 2026Article
- Ethyl methanesulfonate mutagenesis in fungi: genetic mechanisms, applications, and implications for agricultural biotechnology.Archives of microbiology · 2026Review
- Histone Deacetylase 19 Controls Powdery Mildew Susceptibility by Attenuating Biosynthesis of Cuticular Wax and Salicylic Acid.Journal of fungi (Basel, Switzerland) · 2026Article
- Wheat SWI3B Subunit of SWI/SNF Chromatin Remodeling Complex Governs Powdery Mildew Susceptibility by Suppressing Salicylic Acid Biosynthesis.Journal of fungi (Basel, Switzerland) · 2026Article
- From wild shrub to cultivated host: a genetic roadmap for enhancing lac production inFrontiers in plant science · 2026Review
- Wheat DNA Methyltransferase TaMET1 Negatively Regulates Salicylic Acid Biosynthesis to Facilitate Powdery Mildew Susceptibility.Journal of fungi (Basel, Switzerland) · 2025Article
- Chromatin Remodeler TaSWI3D Controls Wheat Susceptibility to Pathogenic FungusMicroorganisms · 2025Article
- Functional analysis of HvSNAC1 in stomatal dynamics and drought adaptation.Journal of applied genetics · 2025Article
- Review
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Wheat Chromatin Remodeling Protein TaSWP73 Contributes to Compatible Wheat-Powdery Mildew Interaction.International journal of molecular sciences · 2025Article
- Wheat Transcriptional Corepressor TaTPR1 Suppresses Susceptibility GenesInternational journal of molecular sciences · 2024Article
- MiRiQ Database: A Platform for In Silico Rice Mutant Screening.Plant & cell physiology · 2024Article
- The ability ofFrontiers in plant science · 2024Article
- Identification of Novel Induced Mutations in Seed and Vegetatively Propagated Plants from Reduced Representation or Whole Genome Sequencing Data.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Identification of Induced Copy Number Variation from Low Coverage Sequence Data.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Microsatellite Markers: A Tool to Assess the Genetic Diversity of Yellow Mustard (Plants (Basel, Switzerland) · 2023Article
- WheatInternational journal of molecular sciences · 2023Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200-500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.