ArticleTransgenic research2012
Production of Phytophthora infestans-resistant potato (Solanum tuberosum) utilising Ensifer adhaerens OV14.
Article in Transgenic research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 65 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
- Atypical rhizobia trigger nodulation and pathogenesis on the same legume hosts.Nature communications · 2024Article
- Article
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- Development of an efficient marker-free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1.Plant biotechnology journal · 2022Article
- Genetic factors governing bacterial virulence and host plant susceptibility duringAdvances in genetics · 2022Review
- Complete genomic sequence and phylogenomics analysis of Agrobacterium strain AB2/73: a new Rhizobium species with a unique mega-Ti plasmid.BMC microbiology · 2021Article
- State of the Art of Genetic Engineering in Potato: From the First Report to Its Future Potential.Frontiers in plant science · 2021Review
- Symbiotic and Nonsymbiotic Members of the Genus Ensifer (syn. Sinorhizobium) Are Separated into Two Clades Based on Comparative Genomics and High-Throughput Phenotyping.Genome biology and evolution · 2020Article
- A robust genetic transformation protocol to obtain transgenic shoots ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Review
- Pathways of DNA Transfer to Plants fromAnnual review of phytopathology · 2019Article
- Insights into the transcriptomic response of the plant engineering bacterium Ensifer adhaerens OV14 during transformation.Scientific reports · 2019Article
- Review
- Article
- Transcriptional Activation of Virulence Genes of Rhizobium etli.Journal of bacteriology · 2017Article
- Agrobacterium-mediated plant transformation: biology and applications.The arabidopsis book · 2017Article
- Transfer of DNA from Bacteria to Eukaryotes.mBio · 2016Review
- Advancing Crop Transformation in the Era of Genome Editing.The Plant cell · 2016Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on the use of Agrobacterium tumefaciens-mediated transformation commodity crop improvement through genetic engineering is the fastest adopted crop technology in the world (James 2010). However, the complexity of the Agrobacterium patent landscape remains a challenge for non-patent holders who wish to generate novel varieties for a commercial purpose. The potential of non-Agrobacterium strains (Transbacter(™)) to modify a plant genome has previously been described. However, they are unlikely to be widely used without significant adjustments in transformation protocols in order to improve their gene transfer efficiencies. In this study we set out to identify alternative bacteria species that could (a) utilize vir genes for genetic transformation and (b) substitute for A. tumefaciens in existing transformation protocols, without a prerequisite for protocol modifications. To this end we isolated a collection (n=751) of plant-associated bacteria from the rhizosphere of commercially grown crops. Based on various screens, including plant transformation with the open-source vector pCAMBIA5105, we identified a strain of the bacterium Ensifer adhaerens with the capacity to transform both Arabidopsis thaliana (0.12%) and potato (mean transformation frequency 35.1%). Thereafter, Ensifer adhaerens was used to generate blight- (causative organism Phytophthora infestans) resistant potato using the Solanum bulbocastanum 'resistance to blight' (RB) gene. Resistant genotypes were confirmed by associated molecular analysis and resistant phenotypes demonstrated by the development of hypersensitive lesions on inoculated leaf tissue post-pathogen inoculation. These data confirm the potential of Ensifer-mediated transformation (EMT) as a novel platform for the high frequency generation of transgenic potato.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.