ArticlePLoS neglected tropical diseases2011
Rapid molecular assays for specific detection and quantitation of Loa loa microfilaremia.
Article in PLoS neglected tropical diseases, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 100 citations in OpenAlex.
- Laboratory diagnosis of loiasis to support individual patient management: A systematic review.PLoS neglected tropical diseases · 2026Pooled it
- The impact of Loa loa microfilaraemia on research subject retention during a whole sporozoite malaria vaccine trial in Equatorial Guinea.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2022Trial
- A Randomized, Placebo-controlled, Double-blind Pilot Study of Single-dose Humanized Anti-IL5 Antibody (Reslizumab) for the Reduction of Eosinophilia Following Diethylcarbamazine Treatment of Loa loa Infection.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Trial
- Clinical and epidemiological features of imported loiasis in Beijing: a report from patients returned from Africa.BMC infectious diseases · 2024Article
- Analysis of diagnostic test outcomes in a large loiasis cohort from an endemic region: Serological tests are often false negative in hyper-microfilaremic infections.PLoS neglected tropical diseases · 2024Article
- A Novel, Highly Sensitive Nucleic Acid Amplification Test Assay for the Diagnosis of Loiasis and its Use for Detection of Circulating Cell-Free DNA.The Journal of infectious diseases · 2023Article
- [Reliability evaluation in rural Congo of the calibrated blood smear technique to assessMedecine tropicale et sante internationale · 2023Article
- Cases of Loiasis Among a Congolese Family: Screening for Loiasis Among Newly Arrived Refugees From Endemic Areas.The journal for nurse practitioners : JNP · 2023Article
- Application of loop mediated isothermal amplification (LAMP) assays for the detection ofFrontiers in tropical diseases · 2023Article
- Longitudinal study of cross-reactive antigenemia in individuals with highFrontiers in parasitology · 2023Article
- Distinct loiasis infection states and associated clinical and hematological manifestations in patients from Gabon.PLoS neglected tropical diseases · 2022Article
- Colorimetric and Real-Time Loop-Mediated Isothermal Amplification (LAMP) for Detection ofDiagnostics (Basel, Switzerland) · 2022Article
- The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper.Journal of tropical medicine · 2022Article
- The Human FilariaResearch and reports in tropical medicine · 2022Review
- Characterising co-infections with Plasmodium spp., Mansonella perstans or Loa loa in asymptomatic children, adults and elderly people living on Bioko Island using nucleic acids extracted from malaria rapid diagnostic tests.PLoS neglected tropical diseases · 2022Article
- Validation of loop-mediated isothermal amplification for the detection of Loa loa infection in Chrysops spp in experimental and natural field conditions.Parasites & vectors · 2021Article
- Sensitive universal detection of blood parasites by selective pathogen-DNA enrichment and deep amplicon sequencing.Microbiome · 2021Article
- Integrating Multiple Biomarkers to Increase Sensitivity for the Detection of Onchocerca volvulus Infection.The Journal of infectious diseases · 2020Article
- Performance of two serodiagnostic tests for loiasis in a Non-Endemic area.PLoS neglected tropical diseases · 2020Article
- Mapping lymphatic filariasis in Loa loa endemic health districts naïve for ivermectin mass administration and situated in the forested zone of Cameroon.BMC infectious diseases · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAccurate diagnosis of Loa loa infection is essential to the success of mass drug administration efforts to eliminate onchocerciasis and lymphatic filariasis, due to the risk of fatal encephalopathic reactions to ivermectin occurring among highly microfilaremic Loa-infected individuals living in areas co-endemic for multiple filarial species. METHODOLOGY/PRINCIPAL
findingsFrom a pool of over 1,800 L. loa microfilaria (mf) expressed sequence tags, 18 candidate L. loa mf-specific PCR targets were identified. Real-time PCR (qPCR) assays were developed for two targets (LLMF72 and LLMF269). The qPCR assays were highly specific for L. loa compared with related filariae and also highly sensitive, with detection limits of 0.1 pg genomic DNA, or 1% of DNA extracted from normal blood spiked with a single L. loa microfilaria. Using various DNA extraction methods with dried blood spots obtained from Cameroonian subjects with parasitologically proven loiasis, the LLMF72 qPCR assay successfully estimated mf burden in 65 of 68 samples (50-96,000 mf/mL by microscopy), including all 12 samples subjected to a simple 10-minute boiling extraction. Additionally, the assay detected low-level microfilaremia among 5 of 16 samples from patients thought to be amicrofilaremic by microscopy. CONCLUSIONS/SIGNIFICANCE: This novel, rapid, highly sensitive and specific qPCR assay is an important step forward in the laboratory diagnosis of L. loa infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.