ArticlePloS one2011
A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 83 citations in OpenAlex.
- Construction of Lentiviral Vectors Carrying Six Pluripotency Genes in Yak to Obtain Yak iPSC Cells.International journal of molecular sciences · 2024Article
- Acquisition and maintenance of pluripotency are influenced by fibroblast growth factor, leukemia inhibitory factor, and 2i in bovine-induced pluripotent stem cells.Frontiers in cell and developmental biology · 2022Article
- Efficient induction and sustenance of pluripotent stem cells from bovine somatic cells.Biology open · 2021Article
- Article
- Strategy to Establish Embryo-Derived Pluripotent Stem Cells in Cattle.International journal of molecular sciences · 2021Review
- Perspectives of pluripotent stem cells in livestock.World journal of stem cells · 2021Review
- Generation of Sheep Induced Pluripotent Stem Cells With Defined DOX-Inducible Transcription FactorsFrontiers in cell and developmental biology · 2021Article
- Generation of induced pluripotent stem cells from large domestic animals.Stem cell research & therapy · 2020Article
- Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos.Journal of stem cells & regenerative medicine · 2020Article
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
- Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system.BMC research notes · 2018Article
- Generation of transgene-free porcine intermediate type induced pluripotent stem cells.Cell cycle (Georgetown, Tex.) · 2018Article
- Naked Mole Rat Induced Pluripotent Stem Cells and Their Contribution to Interspecific Chimera.Stem cell reports · 2017Article
- Pluripotent stem cells and livestock genetic engineering.Transgenic research · 2016Review
- Article
- Inhibition of JAK-STAT ERK/MAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem Cells.Stem cells international · 2016Article
- Cellular reprogramming in farm animals: an overview of iPSC generation in the mammalian farm animal species.Journal of animal science and biotechnology · 2016Review
- Characterization of Bovine NANOG5'-flanking Region during Differentiation of Mouse Embryonic Stem Cells.Asian-Australasian journal of animal sciences · 2015Article
- Derivation and characterization of bovine induced pluripotent stem cells by transposon-mediated reprogramming.Cellular reprogramming · 2015Article
- Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals.World journal of stem cells · 2015Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Authentic induced pluripotent stem cells (iPSCs), capable of giving rise to all cell types of an adult animal, are currently only available in mouse. Here, we report the first generation of bovine iPSC-like cells following transfection with a novel virus-free poly-promoter vector. This vector contains the bovine cDNAs for OCT4, SOX2, KLF4 and c-MYC, each controlled by its own independent promoter. Bovine fibroblasts were cultured without feeders in a chemically defined medium containing leukaemia inhibitory factor (LIF) and inhibitors of MEK1/2 and glycogen synthase kinase-3 signaling ('2i'). Non-invasive real-time kinetic profiling revealed a different response of bovine vs human and mouse cells to culture in 2i/LIF. In bovine, 2i was necessary and sufficient to induce the appearance of tightly packed alkaline phosphatase-positive iPSC-like colonies. These colonies formed in the absence of DNA synthesis and did not expand after passaging. Following transfection, non-proliferative primary colonies expressed discriminatory markers of pluripotency, including endogenous iPSC factors, CDH1, DPPA3, NANOG, SOCS3, ZFP42, telomerase activity, Tra-1-60/81 and SSEA-3/4, but not SSEA-1. This indicates that they had initiated a self-sustaining pluripotency programme. Bovine iPSC-like cells maintained a normal karyotype and differentiated into derivatives of all three germ layers in vitro and in teratomas. Our study demonstrates that conversion into induced pluripotency can occur in quiescent cells, following a previously undescribed route of direct cell reprogramming. This identifies a major species-specific barrier for generating iPSCs and provides a chemically defined screening platform for factors that induce proliferation and maintain pluripotency of embryo-derived pluripotent stem cells in livestock.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.